从甘油中持续生产酸:一个完整的实验和计算研究
Ioannis Zacharopoulos1, Constantinos Theodoropoulos1
1Department of Chemical Engineering, Biochemical and Bioprocess Engineering Group, The University of Manchester, Manchester M13 9PL, UK.
Bioresource technology
|July 22, 2023
概括
一个使用*Actinobacillus succinogenes* (*A. succinogenes*) 连续发酵的新型生物工艺,从甘油中产生了酸. 这项研究实现了创纪录的0.518g/L/h生物糖酸生产率,优化了最大产量的条件.
科学领域:
- 生物技术是生物技术.
- 生物化学工程 生物化学工程
- 工业微生物学 工业微生物学
背景情况:
- 酸是一种有价值的平台化学物质,具有多种工业应用.
- 目前的生产方法往往依赖于石化原料,这引发了对可持续生物基替代品的兴趣.
- 甘油是生物柴油生产的副产品,为微生物发酵提供了丰富且具有成本效益的基质.
研究的目的:
- 开发和优化一种连续的生物工艺,用于从糖醇中生产酸,使用*Actinobacillus succinogenes*.
- 构建和验证用于连续发酵的预测生物工艺模型.
- 确定最佳的操作条件,以最大限度地提高生物糖酸体积生产率和糖转化.
主要方法:
- 开发一个连续的生物反应器系统,用于*A. succinogenes*发酵的细胞回收.
- 在现有双基质限制模型的基础上构建和验证新的生物工艺模型.
- 利用经过验证的模型进行优化研究,以确定最佳的稀释速率,反流速率和料糖醇度.
- 计算帕雷托前线以平衡体积生产率和糖转换.
主要成果:
- 通过一系列操作参数对开发的生物工艺模型进行实验验证.
- 确定产生最大生物酸体积生产率的最佳条件.
- 实现0.518g/L/h的最大体积生产率,这是连续生物工艺的新纪录.
- 在优化条件下证明高糖转化率.
结论:
- 开发的连续生物工艺和验证的模型提供了一种有效的方法,用于从甘油中生产生物甘油酸.
- 该研究成功地确定并通过实验验证了前所未有的酸生产率的最佳条件.
- 这项研究有助于可持续生产酸,利用易于获得的副产品原料.
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