来自Gardenia jasminoides的两个酸性多糖的结构及其潜在的免疫调节活性
Junran Shao1, Ting Li1, Siying Zeng1
1Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China, Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou 510632, China.
International journal of biological macromolecules
|July 22, 2023
概括
来自Gardeniae fructus的两个酸性多糖,GJP50-3和GJP50-4,显示出作为免疫调节剂的潜力. 这些pectin化合物激活巨细胞和调节体外和体内免疫反应,建议在食品和制药中的应用.
科学领域:
- 自然产品化学 自然产品化学
- 免疫学 免疫学 免疫学
- 药理学 药理学是指药理学的学科.
背景情况:
- 果园植物 (Gardeniae fructus) 是一种传统的药草.
- 多糖因其多样化的生物活动而闻名.
- 了解植物化合物的免疫调节潜力对于开发新疗法至关重要.
研究的目的:
- 从Gardeniae fructus中分离和描述酸性多糖.
- 在体外和体内研究这些多糖的免疫调节活性.
主要方法:
- 多糖的分离和净化 (GJP50-3,GJP50-4).
- 使用FT-IR,GC-MS,NMR和凝透色谱进行结构性表征.
- 在体外分析:巨细胞激活 (NO,TNF-α),Luminex液晶片分析 (细胞因子,化学因子).
- 在体内测试:斑马鱼模型 (NO释放,中性粒细胞计数).
主要成果:
- 两个同质的酸性多糖体,GJP50-3 (91.5 kDa) 和GJP50-4 (140.3 kDa),被确定为pectin多糖体.
- 这两种多糖都刺激了氧化 (NO) 的释放和巨细胞中瘤缩因子-α (TNF-α) 的分泌.
- GJP50-3显著增加了各种互白蛋白,TNF-α和化基因的分泌.
- 在体内,GJP50-3和GJP50-4增强了斑马鱼的NO释放和中性粒细胞数量.
结论:
- GJP50-3和GJP50-4具有显著的免疫调节特性.
- 这些多糖体显示出在食品和制药行业作为天然免疫调节剂的使用潜力.
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