在超的压力期间增加细胞内拥挤.
Akira Kitamura1, Sho Oasa2, Haruka Kawaguchi3
1Laboratory of Molecular Cell Dynamics, Faculty of Advanced Life Science, Hokkaido University, Sapporo, Japan. akita@sci.hokudai.ac.jp.
Scientific reports
|July 22, 2023
概括
超的压力迅速改变细胞内巨分子拥挤 (MMC). 测量eGFP光寿命和扩散可提供这些动态细胞变化的早期检测.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 超的压力会诱导细胞过程,包括蛋白质/RNA聚合和相分离.
- 这些由压力引起的细胞内变化的动态和程度尚不清楚.
研究的目的:
- 为了研究活细胞在高度应激下细胞内宏分子拥挤 (MMC) 的动态变化.
- 为了比较eGFP光寿命和扩散的响应时间与福斯特共振能量转移 (FRET) 传感器.
主要方法:
- 使用光终身成像显微镜和光相关谱 (FCS).
- 通过测量单体增强绿色光蛋白 (eGFP) 的光寿命和扩散来量化MMC变化.
主要成果:
- eGFP光寿命对MMC的变化做出了比GimRET FRET传感器更快的反应.
- 在超性压力期间,FCS显示出因MMC升高而增加的细胞内粘度.
- eGFP的生命周期和扩散是细胞内MMC增加的早期指标.
结论:
- 在eGFP光寿命和扩散动态的变化是超体应激诱导的MMC的快速指标.
- 这种方法允许实时观察活细胞内的MMC动态中的过渡状态.
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