使用原子力显微镜与纳米针探针的原子力显微镜进行细胞内纳米结构的实时成像协议
Takehiko Ichikawa1, Mohammad Shahidul Alam2, Marcos Penedo3
1Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
STAR protocols
|July 23, 2023
概括
这项研究介绍了纳米内镜-原子力显微镜 (AFM),这是一种用于活细胞内纳米尺度成像的新技术. 这种方法可以可视化细胞内结构,促进细胞生物学研究.
科学领域:
- 细胞生物学 细胞生物学
- 纳米技术纳米技术
- 显微镜的使用方法
背景情况:
- 原子力显微镜 (AFM) 传统上仅对细胞表面进行成像.
- 在活细胞内部成像纳米结构仍然是一个重大挑战.
研究的目的:
- 介绍纳米内镜-AFM的协议,使活细胞内部的纳米尺度成像成为可能.
- 详细说明使用先进的AFM技术进行细胞内可视化的步骤.
主要方法:
- 细胞染色用于增强成像.
- 制造专门的纳米针探针.
- 2D和3D纳米内镜-AFM用于细胞内观察.
- 3D数据可视化技术. 3D数据可视化技术.
主要成果:
- 在活细胞中成功展示了纳米尺度成像.
- 为纳米内镜-AFM开发一个全面的协议.
- 细胞内纳米结构的可视化.
结论:
- 纳米内镜-AFM提供了一个强大的新工具,用于探索活细胞的内部景观.
- 该协议为了解纳米级细胞过程开辟了新的途径.
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