在某些藻类中固有的CMFDA/FDA染色缺失及其对压载水测试的影响
Gert Hansen1, Gitte Petersen2, Rémi Maguet2
1DHI, Agern Allé 5, DK-2970 Hørsholm, Denmark; Department of Biology, Marine Biological Section, University of Copenhagen, Universitetsparken 4, DK-2100 Copenhagen Ø, Denmark.
Marine pollution bulletin
|July 23, 2023
概括
用于测试压载水系统的重要污点可能会对某些藻类产生错误的负结果. 这可能导致压载水管理系统的有效性评估不准确,可能低估了生物的生存率.
科学领域:
- 海洋生物学 海洋生物学
- 环境科学 环境科学
- 微生物学 微生物学
背景情况:
- 压载水管理系统 (BWMS) 对于防止水生入侵物种至关重要.
- 目前的BWMS疗效测试方法依赖于生物体的移动或CMFDA/FDA等重要污点.
- 一些淡水藻类具有抗性细胞壁,可能会干扰标准测试协议.
研究的目的:
- 调查CMFDA/FDA重要染色的有效性,以检测压载水中的10-50微米生物.
- 评估富含藻原的叶绿素对BWMS测试准确性的影响.
- 为了确定当前BWMS有效性评估中虚假阴性结果的可能性.
主要方法:
- 用CMFDA/FDA评估占主导地位的淡水虫色素的染色率.
- 使用Lugol的固定样本和CMFDA/FDA染色量化10-50μm的生物.
- 调查水晶紫色作为一种潜在的标志物存在的指标.
主要成果:
- 观察到CMFDA/FDA的某些叶绿素的染色率差 (3-70%),表明潜在的假阴性结果.
- 水晶紫色染色给出了对藻类检测的不确定的结果.
- 有机体数量的显著差异 (10,183 / mL与2335 / mL) 强调CMFDA/FDA染色的低估.
结论:
- 由于抗性藻类细胞壁,CMFDA/FDA染色可以在BWMS疗效测试中导致大量虚假阴性 (估计为40-50%) .
- 当前的测试协议可能会高估BWMS的性能.
- 需要进一步的研究来开发更可靠的方法来评估BWMS对各种水生生物的有效性.
更多相关视频
10:20Quantification of Heavy Metals and Other Inorganic Contaminants on the Productivity of Microalgae
Published on: July 10, 2015
16.0K
09:54A Small Volume Bioassay to Assess Bacterial/Phytoplankton Co-culture Using WATER-Pulse-Amplitude-Modulated WATER-PAM Fluorometry
Published on: March 11, 2015
10.6K
相关概念视频
Other Algae
47
The group Stramenopiles include some phototrophic microorganisms. Members of this group possess flagella covered in numerous short, hairlike extensions, a feature that inspired the group's name, derived from the Latin words for "straw" and "hair." Some of the main categories of Stramenopiles include diatoms, golden algae, and brown algae.Diatoms are unicellular, photosynthetic eukaryotes, with over 200 known genera. They play a key role in the planktonic communities of both marine and...
47
Red Algae
59
Red algae, also known as rhodophytes, are primarily found in marine environments, though some species inhabit freshwater and terrestrial ecosystems. These organisms exist in both unicellular and multicellular forms, with some multicellular varieties reaching macroscopic sizes.As phototrophic organisms, red algae contain chlorophyll a; however, their chloroplasts lack chlorophyll b. Instead, they possess phycobiliproteins, which serve as major light-harvesting pigments, similar to those found in...
59
Green Algae
52
Green algae, also referred to as chlorophytes, are different from red algae in having the chloroplasts containing chlorophylls a and b, which give them their distinct green hue. However, they lack phycobiliproteins, preventing them from developing the red or blue-green pigmentation seen in red algae. In terms of photosynthetic pigment composition, green algae closely resemble plants and share a close evolutionary relationship with them. Taxonomically Green algae belong to Phylum Chlorophyta in...
52
Special Staining Techniques
49
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
49
