相关实验视频

Updated: Jul 31, 2026

Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry

Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry

Published on: May 2, 2011

肺内IFN-γ灌注会通过CXCR3通路引起脏和肺部的慢性淋巴细胞炎症

Wenbin Ding¹, Dongting Xu¹, Fengying Li¹

¹State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

International immunopharmacology

|July 23, 2023

概括

肺干扰素- (IFN-γ) 灌注会通过通过IP-10/CXCR3通路调解的脏,血液和肺部T淋巴细胞的增加引起慢性咳.

科学领域:

免疫学免疫学免疫学
呼吸系统医学呼吸系统医学
细胞生物学细胞生物学

背景情况:

慢性难治性咳与高肺干扰素- (IFN-γ) 和IFN-γ产生T淋巴细胞有关.
肺部IFN-γ可以引起急性炎症和咳,但其在慢性疾病和T淋巴细胞增殖中的作用尚不清楚.

研究的目的:

通过刺激脏中IFN-γ产生T淋巴细胞的增殖,研究肺部IFN-γ是否会诱导慢性气道炎症和咳过敏.

主要方法:

小鼠接受了肺部IFN-γ的使用.
评估脏重量,淋巴细胞增殖 (体内和体外),以及脏,血液和肺部的IP-10和CXCR3+T淋巴细胞水平.
使用AMG487,一种IP-10/CXCR3抑制剂,以阻止炎症途径.

主要成果:

肺部IFN-γ诱导的慢性气道炎症和咳过敏.
在脏,血液和肺部发现IFN-γ产生T淋巴细胞的增加.
IFN-γ的使用促进了脏淋巴细胞的增殖,增加了脏和肺部的IP-10和CXCR3+T细胞.
在实验室中,IP-10促进了脏淋巴细胞的增殖.
AMG487阻断了IFN-γ诱导的炎症和T淋巴细胞的增殖.

结论:

肺内IFN-γ灌注通过IP-10/CXCR3通路刺激脏IFN-γ产生T淋巴细胞的增殖.
这些淋巴细胞从脏释放到血液中,由肺部IP-10通过CXCR3招募到肺部.
肺部IFN-γ产生T淋巴细胞和IFN-γ有助于慢性气道炎症和咳过敏.

关键词:

在CXC化学因子受体3 慢性难治性咳慢性难治性咳干扰因子-γ诱导蛋白 10 干扰因子-γ诱导蛋白干扰素-γ 干扰素-γ 的作用脏脏是指脏的部分.

更多相关视频

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy

Published on: April 16, 2019

Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation

Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation

Published on: July 6, 2019

相关概念视频

Chronic Inflammation: Introduction

Chronic Inflammation: Introduction

Chronic inflammation is a prolonged, dysregulated immune response that persists for weeks to years when the inciting stimulus is difficult to eradicate or when self‑antigens drive ongoing reactivity. Morphologically, it is defined by mononuclear cell infiltration, progressive tissue destruction, and concurrent attempts at healing via angiogenesis and fibrosis. Compared with acute inflammation, edema is less prominent while cellular infiltration predominates; triggers include persistent...

Inflammatory Bowel Disease III: Crohn's Disease

Inflammatory Bowel Disease III: Crohn's Disease

Crohn’s disease is a chronic, relapsing form of inflammatory bowel disease characterized by segmental, transmural inflammation that can affect any part of the gastrointestinal tract. Its pathogenesis arises from a combination of genetic susceptibility, environmental exposures, epithelial barrier dysfunction, and immune dysregulation. Together, these factors lead to an exaggerated immune response against components of the gut microbiome.Genetic and Environmental InfluencesMultiple genetic...