洞察抗生素耐药性基因丰度和在堆肥过程中由离子液体诱导的调节机制
Hongxiang Yang1, Heng Lu1, Kecheng Li1
1School of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, China.
Journal of environmental management
|July 23, 2023
概括
在堆肥过程中,离子液体 (IL) 的预处理有效地降低了抗生素耐药性基因 (ARG). 这种方法抑制了宿主细菌并改变了代谢途径,减少了ARGs.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 抗生素耐药性基因 (ARG) 构成重大环境和公共卫生风险.
- 堆肥是一种常见的废物处理方法,但它对ARG的影响需要进一步研究.
- 离子液体 (ILs) 正在成为废物处理和生物修复的潜在剂.
研究的目的:
- 通过使用离子液体 (ILs) 预处理,研究在粉和牛堆肥过程中ARG的调节机制.
- 在ILs治疗下量化特定ARG (MLS,氨基醇,四环素,β-乳糖) 的减少.
- 阐明ILs影响ARG减少的机制.
主要方法:
- 粉和牛的堆肥,有或没有ILs预处理.
- 使用分子方法对ARG进行定量分析.
- 对细胞外聚合物质 (EPS) 和宿主细菌种群的分析.
- 代谢分析以确定受影响的途径.
主要成果:
- 在堆肥过程中,ILs的预处理显著降低了MLS,氨基醇,四环素和β-乳ARG的丰度.
- 观察到的特异性降低:MLS (25.62% CK, 26.66% T),四环素 (7.21% CK, 7.86% T),氨基醇 (2.85% CK, 3.34% T),β-乳酸胺 (1.95% CK, 3.69% T). 在这种情况下,该药物可能会被视为一种药物.
- ILs促进了EPS的分解,增强了乳糖脱酶 (LDH) 的释放,抑制了特定的ARG宿主 (例如Firmicutes),并降低了ARG传播途径的调节.
结论:
- 在堆肥过程中,ILs的预处理是减少ARG的一个有希望的策略.
- 该机制涉及EPS降解,宿主抑制和ARG传播途径的下调.
- 这种方法有助于缓解环境中抗生素耐药性的传播.
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