植物DNA信息在确定未知受害者的来源和身份方面的潜力
Qing Chen1, Mengyan Liu2, Chao Xu3
1China University of Political Science and Law, Beijing 100091, China; Forensic Science Service of Beijing Public Security Bureau, Beijing 100092, China.
Forensic science international
|July 23, 2023
概括
来自肺组织的植物DNA可以在刑事案件中识别未知的受害者. 这种法医方法使用花粉颗粒来缩小地理来源,在其他证据稀缺时有助于识别受害者.
科学领域:
- 法医科学 法医科学 法医科学
- 帕利诺学 (Palynology) 是一个临床学科.
- 环境DNA (eDNA) 分析
背景情况:
- 确定受害者的身份对于刑事调查至关重要,但识别信息往往有限.
- 在人类肺组织中发现的植物颗粒,特别是花粉,可以提供地理线索.
- 环境DNA (eDNA) 分析为法医识别提供了一种新的方法.
研究的目的:
- 调查从人类肺组织中提取的植物花粉DNA的潜力,以确定受害者的来源.
- 评估使用植物eDNA来缩小未知受害者地理搜索范围的可行性.
- 在法医调查中展示植物eDNA的实际应用.
主要方法:
- 从155位死亡的死亡原因不同的人身上收集肺部组织.
- 从肺组织中提取DNA,然后使用Illumina Hiseq平台对植物DNA片段进行测序.
- 遗传学条形码识别植物物种及其地理分布.
主要成果:
- 在55个样本中,从36个物种中确定了108个独特的植物序列.
- 在16个样本中检测到主要由昆虫授粉的作物和装饰植物,以及21种野生植物物种.
- 在真实案例中成功应用,将未知尸体的来源缩小到特定的省份,并确认身份.
结论:
- 肺组织中的植物颗粒 (花粉) 作为确定受害者来源的宝贵法医证据.
- 植物eDNA分析可以显著缩小对未知受害者的搜索范围,特别是在有限的物质证据的情况下.
- 一个全面的本地参考库对于准确识别植物物种和有效的法医应用至关重要.
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