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RNA螺旋酶DExD/H-box 5调节小鼠的肠道微生物群

Liyuan Liu¹, Silan Zhang², Feijie Zhi³

¹College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei Province, 071001, China; State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, 730046, China.

Microbial pathogenesis

|July 23, 2023

概括

RNA螺旋酶DDX5蛋白质影响小鼠肠道微生物群的组成. 灭绝DDX5的小鼠表现出改变的微生物结构,这表明它们在肠道健康和疾病中的作用.

科学领域:

微生物学微生物学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

DExD/H-box (DDX) 类RNA酶家族对于细胞RNA代谢至关重要.
DDX5 (p68) 与病毒复制和瘤发生有关.
对于DDX蛋白在调节肠道微生物群中的作用仍然未被探索.

研究的目的:

为了研究DDX5对肠道微生物群组成的影响.
建立和分析DDX5淘汰赛小鼠 (Ddx5^+/-) 的微生物群变化.
探索DDX5作为肠道炎症和瘤发生的潜在治疗点.

主要方法:

使用CRISPR/CAS9技术生成了Ddx5^+/-小鼠.
从Ddx5^+/-和野生类型 (Ddx5^+/+) 的小鼠收集了便和骨样本.
进行了16S rRNA测序和分析,以评估微生物多样性,丰富性和结构.

主要成果:

Ddx5^+/-小鼠成功地产生了正常的阴茎形态.
没有观察到总体微生物多样性和丰度的显著差异.
在Ddx5^+/+和Ddx5^+/-小鼠之间发现了微生物结构的显著聚类.
在Ddx5^+/-小鼠中,Akkermansia和Clostridium_sensu_stricto_1的相对丰度明显较低.

结论:

DDX5显著影响小鼠肠道微生物群的组成.
肠道微生物群和DDX5蛋白之间存在特定的相互作用.
DDX5是治疗肠道炎症和瘤发生的潜在新目标.

关键词:

在阿克克曼斯的阿克克曼西亚.克洛斯特里迪亚_sensu_stricto_1 的时间我们的肠道微生物群.我们的老鼠是老鼠.RNA直酶 DExD/H-盒子 5 在

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