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基于高通量测序检测日本脑炎病毒及其对微型核糖核酸的影响

Qinghua Liu¹, Weisheng Deng¹, Xuemin Guo²

¹Department of Neurology 2, Meizhou People's Hospital, Meizhou, 514031, Guangdong Province, China.

Microbial pathogenesis

|July 23, 2023

概括

日本脑炎病毒 (JEV) 感染显著降低了小鼠大脑组织和神经细胞中的微RNA (miRNA) 表达. 这项研究确定了受到JEV影响的特定miRNA,为病毒机制提供了洞察力.

科学领域:

神经科学是一个神经科学.
病毒学病毒学
分子生物学分子生物学

背景情况:

日本脑炎病毒 (JEV) 是一个重要的神经向性病原体.
微RNAs (miRNAs) 在调节基因表达和细胞过程中起着至关重要的作用.
了解JEV和宿主miRNA之间的相互作用对于阐明病毒病原性至关重要.

研究的目的:

研究JEV感染对宿主细胞和小鼠大脑组织中miRNA表达特征的影响.
为了确定在JEV感染期间差异表达的特定miRNAs.
探索与miRNA失调相关的JEV病变的潜在机制.

主要方法:

使用高通量测序分析了JEV感染小鼠大脑组织和神经元细胞系 (BV2,NA,bEnd.3) 中的miRNA表达.
在不同的感染多重度 (MOI) 上,对细胞系的试验性感染JEV.
RNA提取和阿加罗斯凝电泳,以确认RNA的完整性.

主要成果:

感染JEV导致小鼠大脑组织和神经元细胞中整体miRNA表达的显著下调.
在小鼠大脑组织中,有2986个miRNA被下调和1251个被上调.
特定的miRNAs,包括miR-466d-3p和miR-381-3p,在感染JEV的细胞中显示出显著的下调调节.

结论:

简核病毒感染深刻地改变了宿主细胞的miRNA格局,主要是通过下调.
已识别的下调 miRNAs 可能是宿主对 JEV 感染的反应的关键参与者.
这些发现有助于了解JEV病原体,并可能提供潜在的治疗点.

关键词:

检测检测检测检测检测高通量测序的高通量测序日本脑炎病毒病毒.机理机制机理机制微核糖核酸是一种微核糖核酸.

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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...