急性酒精和慢性饮酒双向调节前额叶皮层血管活性肠内部神经元的刺激性
Shannon M Thompson1, Carly B Fabian2, Anthony S Ferranti3
1Department of Psychiatry, University of Pittsburgh, Pittsburgh, PA, 15219, USA; Translational Neuroscience Program, University of Pittsburgh, Pittsburgh, PA, USA.
Neuropharmacology
|July 23, 2023
概括
急性酒精增强了前额叶皮层 (PFC) 血管活性肠 (VIP) 内神经元刺激性. 慢性饮酒会降低VIP-内部神经元的刺激性,在禁酒后男性有持久的影响.
科学领域:
- 神经科学是一个神经科学.
- 酒精使用障碍研究研究
背景情况:
- 前额叶皮质 (PFC) 对于调节饮酒行为和对慢性酒精暴露的情绪反应至关重要.
- 在PFC内部的局部抑制性内内神经元 (INs) 调节其活性,不同的亚型如parvalbumin, somatostatin和血管活性肠 (VIP) INs发挥特定的作用.
- 虽然在酒精模型中研究了帕瓦胺和体静止素INs的适应,但对VIP INs的影响在很大程度上是未知的.
研究的目的:
- 研究急性乙醇和慢性饮酒对PFC VIP INs的生理影响.
- 确定VIP IN中观察到的变化是否持续到戒断.
主要方法:
- 全细胞补丁电生理学被用来记录从基因工程小鼠的VIP INs.
- 实验涉及急性应用乙醇到大脑切片和慢性间歇性访问 (IA) 乙醇4周.
- 在禁欲期 (7-13天) 之后,还进行了电生理学记录.
主要成果:
- 急性乙醇应用显著提高了PFC VIP INs的兴奋性.
- 经过4周的IA乙醇,雄性和雌性小鼠中的VIP INs与对照组相比,刺激能力降低.
- 这种低刺激性在禁食7-13天后在雄性小鼠中持续存在,但在雌性小鼠中没有.
结论:
- 急性乙醇暴露会增加PFC VIP IN的兴奋性.
- 慢性乙醇消费诱导PFC VIP INs的恒温适应,导致刺激性降低.
- 这些适应性表现出性别特异性的禁欲持久性,突出了酒精对大脑电路的长期影响中的潜在性别差异.
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