在原发性Sjogren综合征中进行蛋白质组学分析和氨酸化分析
Zhennan Liao1, Dandan Li2, Shengyou Liao2
1Department of Nephrology, Institute of Nephrology and Blood Purification, The First Affiliated Hospital of Jinan University, Guangzhou, China; China Clinical Medical Research Center, Guangdong Provincial Engineering Research Center of Autoimmune Disease Precision Medicine, Shenzhen Engineering Research Center of Autoimmune Disease, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, China.
Journal of proteomics
|July 23, 2023
概括
在初级Sjogren中氨酸-氨酸化 (Kmal).
科学领域:
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 主要Sjogren综合征 (pSS) 是一种慢性自身免疫性疾病,其原因尚不清楚.
- lysine-malonylation (Kmal),一种新的翻译后修饰,与代谢,免疫和炎症过程有关.
研究的目的:
- 研究Kmal在pSS中的蛋白质结构和功能作用.
- 与健康对照人群相比,在pSS患者中识别差异表达和结蛋白质.
主要方法:
- 基于液体染色学-双重质谱学 (LC-MS/MS) 的蛋白质组学.
- 来自28名pSS患者和27名健康对照者的蛋白质组数据的生物信息学分析.
主要成果:
- 在pSS中确定了331个下调调节的蛋白质和289个上调调节的蛋白质.
- 观察到TGFB1和CD40LG的下调,以及STAT1.1的上调.
- 在蛋白质中发现了差异性的Kmal修饰,包括在焦点粘附路径中丰富的整合素结合激酶 (ILK).
结论:
- 降低TGFB1和CD40LG的调节有助于pSS炎症.
- STAT1上调与IL-27免疫力和pSS免疫功能障碍有关.
- 通过对ILK的Kmal修改,可以通过对焦粘附路径的调节促进pSS的发病.
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