对焦微型X射线光分析用于结构化和不均样本的非破坏性检测
Korbinian Heimler1, Christine Gottschalk1,2, Carla Vogt3
1Institute of Analytical Chemistry, TU Bergakademie Freiberg, Leipziger Str. 29, 09599, Freiberg, Germany.
Analytical and bioanalytical chemistry
|July 23, 2023
概括
同焦微X射线光 (CMXRF) 谱学提供非破坏性,深度分辨率的元素分析. 这种技术对于分析材料科学,地质学,生物学,艺术和考古学等复杂样品具有价值.
科学领域:
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
- 物理 物理学 物理
背景情况:
- 同焦微型X射线光谱 (CMXRF) 是一种强大的分析技术.
- 它提供非破坏性,深度解析和元素特异性的元素组成分析.
- 该技术利用聚焦的X射线来激发样本原子,然后这些原子发出可检测的光辐射.
研究的目的:
- 详细介绍Confocal微型X射线光学 (CMXRF) 光谱学的原理和应用.
- 突出其在深度解析和元素特定分析方面的能力.
- 讨论它在各种科学和文化遗产领域的实用性.
主要方法:
- 采用一束聚焦的单色或多色X射线来激发样本原子.
- 使用聚焦毛细血管光学来检测发射的光辐射.
- 通过定义特定的探测体积,利用共聚焦设计进行微米分辨率的深度解析分析.
主要成果:
- 通过CMXRF光谱,可以在定义的样本体积内确定元素的存在和度.
- 这种技术对于具有不均元素分布和光矩阵的样本特别有效.
- 通过在空间坐标上映射光强度,可以生成三维元素分布图像.
结论:
- CMXRF光谱是一种多功能工具,用于分析各种领域的元素组成,包括材料科学,地质学,生物学,艺术和考古学.
- 它的非破坏性和深度解决性质为样品组成和结构提供了独特的见解.
- 预计正在进行的技术进步将扩大CMXRF光谱的应用范围,提高3D分析中的灵敏度和量化.
相关概念视频
Confocal Fluorescence Microscopy
13.4K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.4K
X-ray Diffraction of Biological Samples
3.9K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
3.9K
Super-resolution Fluorescence Microscopy
7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K
Scanning Electron Microscopy
4.3K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Fundamental Principles
Accelerated...
4.3K
Electron Microscope Tomography and Single-particle Reconstruction
2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K


