两种富血小板血吸收技术对使用双注射器引力离心系统的血细胞度的影响
Rachel L Jones1, Steven W Frederick1, Alan R Cross1
1BluePearl Specialty and Emergency Pet Hospital, Sandy Springs, Georgia.
Veterinary surgery : VS
|July 24, 2023
概括
闪光方法不需要生产富血小板血 (PRP). 闪光和预闪光方法都产生PRP,但避免色外套将不必要的白细胞和红细胞降到最低.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 生物技术是生物技术.
- 血液学 血液学 血液学
背景情况:
- 富于血小板的血 (PRP) 用于其再生性质.
- 优化PRP制备对于治疗疗效至关重要.
- 了解吸收技术会影响细胞组成.
研究的目的:
- 为了比较吸入技术来最大限度地提高PRP中的血小板度.
- 确定在PRP中尽量减少白细胞和红细胞污染的方法.
- 评估"闪光"技术与使用双注射器系统的"预闪光"方法.
主要方法:
- 在对30只成年狗进行了一项受控的实验室研究.
- 整体血液使用自主调节血 (ACP) 注射器和EDTA管进行处理.
- 两种吸血技术 (预闪和闪) 进行了比较,并对所有样品进行了全血计.
主要成果:
- 与闪光前方法 (3.3 × 105/dL) 相比,闪光方法的平均血小板度显著更高 (4.1 × 105/dL).
- 闪光前样本显示白细胞 (0/dL) 和红细胞 (0%) 的中位数度明显低于闪光样本.
- 对照样本的血小板度最低,闪光样本的血小板,白细胞和红细胞水平最高.
结论:
- "闪光"技术对于生成PRP并不重要.
- "预闪"和"闪"方法都能成功地产生PRP.
- 临床医生应避免泡外套吸收,以尽量减少白细胞和红细胞污染在PRP的特定疗法.
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