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相关概念视频

Translesion DNA Polymerases02:10

Translesion DNA Polymerases

10.0K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
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The Replisome03:01

The Replisome

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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
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DNA Bacteriophages01:26

DNA Bacteriophages

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Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
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Chromosome Structure02:40

Chromosome Structure

22.9K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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Replication in Prokaryotes02:35

Replication in Prokaryotes

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Overview
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相关实验视频

Updated: Jul 22, 2025

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
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In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

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抗选择性自我复制剂

Shuo Yang1,2,3, Yannick Geiger2, Marc Geerts2

  • 1State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.

Journal of the American Chemical Society
|July 24, 2023
PubMed
概括
此摘要是机器生成的。

这对生命的起源至关重要. 研究人员开发出一种能分类性前体的自复制剂,从而推进了生命的起源研究.

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Chemical Triphosphorylation of Oligonucleotides
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Chemical Triphosphorylation of Oligonucleotides

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Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
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Last Updated: Jul 22, 2025

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In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

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Chemical Triphosphorylation of Oligonucleotides
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Chemical Triphosphorylation of Oligonucleotides

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Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
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科学领域:

  • 化学学
  • 生命起源的研究
  • 超分子化学

背景情况:

  • 它们是了解生命起源的关键.
  • 奇拉性对生命至关重要,但它在早期自我复制中的作用还未得到充分研究.

研究的目的:

  • 研究性对自我复制分子的影响.
  • 为了研究生命的出现,研发出反选择性自我复制剂.

主要方法:

  • 合成自组装的自我复制器.
  • 评估了对抗选择性和前体的结合.
  • 研究了对抗选择性的结构影响.

主要成果:

  • 开发了新的自我组装自我复制器,表现出自发的反选择性.
  • 实现了性前体的高效分类,使其成为纯复制体.
  • 确定结构特征 (环大小) 控制对抗选择性.

结论:

  • 自选性自我复制剂可以从纯净的材料中出现和生长.
  • 这些复制器可以根据性对血细胞前体进行分类.
  • 结构约束在自我复制者的反选择性中起着至关重要的作用,促进了生命的新生合成.