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在昆虫养和繁殖中对中性脂酶的功能分析

Hai-Bin Lu¹, Jia-Bao Lu¹, Li-Li Li¹

¹State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China.

Pest management science

|July 24, 2023

概括

中性脂酶对豆虫Riptortus pedestris至关重要,影响其繁殖和生存. 准这些脂酶为控制这种重要的大豆害虫提供了一个有希望的RNAi战略.

科学领域:

昆虫学昆虫学是一门学科.
分子生物学分子生物学
生物化学生化学

背景情况:

豆虫 (Riptortus pedestris) 由于其种子养习惯,导致大豆作物大幅损失.
其适应脂质丰富的大豆种子背后的分子机制尚不清楚.
了解这些机制是制定有效的害虫控制策略的关键.

研究的目的:

在Rpptortus pedestris中功能性地表征中性脂质.
为了确定基于RNA干扰 (RNAi) 的害虫控制的潜在目标基因.
研究酶在昆虫养,繁殖和生存中的作用.

主要方法:

在R. pedestris中识别和基因分析了69个脂质酶,重点是35个扩展的中性脂质酶.
在唾液腺,肠道和卵巢中分析脂酶表达模式.
使用RNAi. 选择特定中性脂酶基因的功能分析.

主要成果:

总共有69个形被确定,其中包括35个中性形的扩张.
发现三个卵巢特异性中性脂酶 (Chr1.3195,Chr1.0994,Chr5.0087) 对于昆虫的繁殖至关重要.
两种肠道特异性中性脂酶 (Chr4.0221,Chr1.3207) 影响了昆虫的生存和体重增加,而唾液腺特异性脂酶没有显著影响.

结论:

特定的中性脂酶 (Chr1.3195,Chr1.0994,Chr5.0087,Chr4.0221,Chr1.3207) 对R. pedestris的食和繁殖至关重要.
这种昆虫对这些中性脂酶的缺陷具有很高的敏感性.
中性脂酶代表了基于RNAi控制大豆种植中的R. pedestris的有希望的目标.

关键词:

这是一种RNA干扰.里普托尔图斯 (Riptortus pedestris) 是一个步行者.养养养养化过程中发生的化.生殖生殖的繁殖.

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