膀癌细胞和纤维细胞之间的依赖HGF的正反循环介导淋巴血管生成和淋巴转移
Yuting Li1, Hanhao Zheng2,3, Yuming Luo4
1Department of Oncology, Sun Yat-sen Memorial Hospital, Guangzhou, Guangdong, P. R. China.
Cancer communications (London, England)
|July 24, 2023
概括
这项研究揭示了LINC00665如何通过激活癌症相关纤维细胞 (CAF) 来促进膀癌 (BCa) 淋巴结转移. 针对LINC00665提供了转移性BCa的潜在治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 与癌症相关的纤维细胞 (CAFs) 通过与癌细胞的相互作用驱动瘤的进展.
- 瘤衍生的细胞外囊泡 (EVs) 介导癌症和 stromal 细胞之间的通信,促进转移.
- 在膀癌 (BCa) 淋巴结 (LN) 转移中,EV介导CAF激活的机制在很大程度上仍未被探索.
研究的目的:
- 研究瘤衍生的EVs在调节CAFs表型中的作用.
- 阐明CAFs表型影响BCa LN转移的分子机制.
- 为了确定驱动BCa LN转移的EV-CAF交叉语音中的关键介质.
主要方法:
- 高通量测序以识别与CAF相关的长非编码RNA (lncRNAs).
- 在体外和体内测试以评估LINC00665介导的EV对纤维细胞到CAF过渡的功能作用.
- 染色体测定,细胞因子分析和患者衍生异种移植 (PDX) 模型,以探索潜在的机制.
主要成果:
- CAFs富含BCa,与淋巴血管生成和LN转移相关.
- LINC00665是一种新型的lncRNA,它调解了CAF在BCa中的透,并与LN转移和不良预后有关.
- LINC00665上调RAB27B,促进EV分泌和CAF表型,建立RAB27B-HGF-c-Myc反循环,增强BCa淋巴血管生成和LN转移.
结论:
- LINC00665通过涉及CAF的RAB27B-HGF-c-Myc反循环驱动BCa LN转移.
- 阻断EV传播的LINC00665或HGF可以抑制BCa淋巴血管生成.
- LINC00665代表了LN转移性BCa.的潜在治疗标.
关键词:
与癌症相关的纤维细胞.在HGF的基础上.膀癌:膀癌是一种癌症.细胞外囊泡中的细胞外囊泡.长非编码RNA是什么意思淋巴结转移是淋巴结的转移.淋巴血管生成 (Lymphangiogenesis) 是一种导致淋巴血管发生的过程.积极的反循环是一个循环.更多相关视频
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