在艾滋病毒/结核病同时感染的外周细胞因子分泌细胞反应的表征
Yuting Tan1,2, Wei Guo3,4, Qi Zhu5
1Department of Infectious Diseases, Zhongnan Hospital of Wuhan University, Wuhan, China.
Frontiers in cellular and infection microbiology
|July 24, 2023
概括
人类免疫缺陷病毒 (HIV) 和结核病 (TB) 的同时感染会改变外围细胞因子分泌细胞. 瘤坏死因子-α (TNF-α) 分泌的CD8+ T细胞可能表明同时感染的患者的早期治疗反应.
科学领域:
- 免疫学 免疫学 免疫学
- 传染性疾病 传染性疾病
- 细胞生物学 细胞生物学
背景情况:
- 人类免疫缺陷病毒 (HIV) 和结核病 (TB) 联合感染的免疫反应是复杂的,并未完全理解.
- 边缘细胞因子分泌细胞反应对于治疗共同感染至关重要,但需要进一步阐明.
研究的目的:
- 在HIV/TB同时感染中,研究外围的促炎,调节和细胞毒性细胞因子分泌细胞的功能特征.
- 将这些概况与单独结核病和艾滋病毒感染的概况进行比较.
- 评估抗结核治疗对这些细胞群的影响.
主要方法:
- 直接细胞内细胞因子染色 (ICS) 和流动细胞计用于分析细胞因子分泌细胞.
- 测量了各种细胞因子分泌细胞 (IFN-γ,TNF-α,IL-17A,TGF-β,穿孔素,大酶A) 的绝对数量和百分比.
- 患者与艾滋病毒/结核病联合感染 (HT),结核病,艾滋病毒,和健康的对照 (HC) 被录取和比较,与抗结核治疗后的随访.
主要成果:
- 与单独结核病或艾滋病毒相比,艾滋病毒/结核病联合感染显示了关键细胞因子分泌细胞的绝对数量明显较低.
- 两组之间观察到CD4+,CD8+T细胞和分泌各种细胞因子的CD14+单细胞的百分比有明显的差异.
- 经过一周的抗结核治疗后,在HIV/TB同时感染组中,TNF-α分泌CD8+ T细胞的百分比增加.
结论:
- 与单个感染相比,HIV/TB联合感染显著改变了外围细胞因子分泌细胞的特征.
- 瘤坏死因子-α (TNF-α) 分泌的CD8+ T细胞可以作为一种敏感的标记物,用于早期评估HIV/TB同时感染患者的抗结核治疗疗效.
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