由Fusobacterium nucleatum和Streptococcus spp.造成的肺部. 同时感染mNGS:一个病例系列
Na Wang1,2, Zhichao Gao1,3, Shuai He4
1Department of Breast Medicine, Cancer Hospital of China Medical University, Liaoning Cancer Hospital, Shenyang, 110042, China.
Open life sciences
|July 24, 2023
概括
大基因组下一代测序 (mNGS) 在培养负肺中准确识别了病原体. 这种先进的技术为诊断肺部感染和指导向治疗策略提供了一个有希望的替代方案.
科学领域:
- 传染性疾病 传染性疾病
- 微生物学 微生物学
- 基因组学就是基因组学.
背景情况:
- 肺部是死性肺部感染,常常难以通过常规培养来诊断.
- 目前用于肺的诊断方法经常导致假阴性,需要经验性抗生素治疗.
- 经验广泛的抗生素使用有助于抗菌素耐药性,并可能使患者的病情恶化.
研究的目的:
- 评估元基因组下一代测序 (mNGS) 作为培养负肺的诊断工具.
- 在没有对传统培养方法反应的肺患者中识别微生物病原体.
- 评估mNGS在指导肺的向治疗中的有用性.
主要方法:
- 研究了三个患有培养负肺的患者.
- 临床表现包括发烧,咳,呼吸困难和胸痛,CT扫描证实肺部病变.
- 支气管支气管洗和胸腔液样本使用元基因组下一代测序 (mNGS) 进行了分析,用于病原体识别.
主要成果:
- 在mNGS中发现了*Fusobacterium nucleatum*和*Streptococcus* spp. (*S. constellatus*, *S. intermedius*) 作为主要的病原体.
- 该研究表明mNGS作为一种可行的替代方案,用于检测肺部的病原体的能力.
- 病原体的鉴定强调了 *Streptococcus* 属在共感染中的重要性.
结论:
- 大基因组下一代测序 (mNGS) 是一种强大的诊断工具,用于识别培养负肺的病原体.
- 与传统培养方法相比,mNGS提供了更全面的病原体概况.
- 针对基于mNGS结果的特定属 (如*Streptococcus*),可以改善肺副感染的治疗策略.
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