使用网络药理方法和实验验证实验验验证,探索桑格瓜饮料在db/db小鼠中对2型糖尿病的保护作用
Yu Cai1, Simin Liu1, Fei Zeng1
1Hubei Provincial Research Center for TCM Health Food Engineering, School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, China.
Heliyon
|July 24, 2023
概括
桑瓜饮料 (SGD) 在治疗2型糖尿病 (T2DM) 中表现有前途. 它通过激活小鼠的关键信号通路来改善胰岛素抵抗和葡萄糖代谢.
科学领域:
- 传统中国医药 传统中国医药
- 药理学 药理学是指药理学的学科.
- 代谢疾病 代谢疾病
背景情况:
- 2型糖尿病 (T2DM) 是一个全球性的健康挑战.
- 桑瓜饮料 (SGD) 是用于T2DM治疗的传统配方.
- 了解SGD的作用机制对于其临床应用至关重要.
研究的目的:
- 调查T2DM中SGD的活性成分和作用机制.
- 探索潜在的治疗点和参与SGD影响的信号通路.
- 通过体内实验来验证发现.
主要方法:
- 组件目标和目标途径分析的网络药理方法.
- 使用TargetNet和PharmMapper进行目标预测.
- 使用Cytoscape构建的蛋白质-蛋白质相互作用 (PPI) 和组件-目标-通路 (C-T-P) 网络.
- 在T2DM小鼠模型 (db/db小鼠) 中进行了体内实验.
主要成果:
- 从SGD中确定了54种化合物和41种关键标蛋白.
- 揭示了98个可能由SGD调节的丰富信号通路.
- 在T2DM小鼠中,SGD干预显著增加了p-AMPK,p-ACC和p-AKT水平.
- 在T2DM小鼠中,SGD干预显著降低了FOXO1水平.
结论:
- 在T2DM中,SGD可能会改善胰岛素抵抗和葡萄糖代谢.
- 该机制涉及AMPK/Akt信号通路的激活.
- SGD显示出作为2型糖尿病治疗剂的潜力.
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