相关实验视频

Updated: Jul 22, 2025

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

Published on: July 13, 2018

甲型玻丁毒素通过调节低氧/低氧化处理的内皮细胞中的内网压力来激活保护性自

Yinhua Zhao¹, Huang Lin¹, Jiaxing Ma¹

¹Plastic and Reconstructive Surgery, Beijing Anzhen Hospital, Capital Medical University, Chaoyang District, Beijing, 100029, China.

|July 24, 2023

概括

甲型玻尿素毒素 (BTXA) 通过诱导中度内质网膜 (ER) 应激和自,保护人类皮肤微血管内皮细胞免受缺血/再损伤. 这种细胞反应增强了细胞的生存和完整性,这表明了治疗点.

科学领域:

细胞生物学细胞生物学
分子医学是分子医学.
伤口治愈研究研究伤口治愈研究

背景情况:

毒素A型 (BTXA) 通过诱导自而显示出对皮肤片中缺血/反 (I/R) 损伤的保护作用.
在BTXA对人类皮肤微血管内皮细胞 (HDMECs) I / R损伤的保护机制中,内质网膜 (ER) 应激和自激活的作用仍然未被证明.

研究的目的:

调查ER应力自激活在BTXA介导的HDMECs对低氧和再氧化 (H/R) 损伤的保护中的作用.
确定最佳BTXA度的细胞保护及其对ER压力和自标志物的影响.

主要方法:

在H/R暴露之前,HDMECs被预处理了BTXA度的变化.
使用细胞计数套件8 (CCK8) 评估了细胞活力.
分析了ER压力 (GRP78,CHOP) 和自 (LC3II/I,Beclin-1) 标记者的蛋白质表达,通过西方斑点 (WB).
评估了ER压力调节剂 (Thapsigargin,4-phenylbutyrate) 和自抑制剂 (Bafilomycin A1) 的作用.

主要成果:

H/R治疗显著增加了ER压力和自标志物.
最佳的BTXA预处理 (1.6 U/mL) 增强了细胞存活率和标记物表达.
ER压力诱导剂/抑制剂和自抑制剂降低了BTXA的保护作用和调节的标记物表达.
由BTXA引起的中度ER压力会触发保护性自.

结论:

BTXA预处理通过诱导中度ER压力来保护HDMECs免受H/R损伤,这反过来又激活了保护性自.
过度的ER压力及其完全抑制都会否定BTXA的细胞保护作用.
适度的ER压力和自对细胞寿命和功能完整性至关重要,呈现出潜在的治疗策略.

关键词:

自自是一种自的过程.毒素A类型的一种毒素.细胞内膜网膜应激压力人体皮肤微血管内皮细胞低氧/低氧化/低氧化

更多相关视频

Activating Autophagy by Aerobic Exercise in Mice

Activating Autophagy by Aerobic Exercise in Mice

Published on: February 3, 2017

Evaluating Cell Death Signaling by Immunofluorescence in a Rat Model of Ischemic Stroke

Evaluating Cell Death Signaling by Immunofluorescence in a Rat Model of Ischemic Stroke

Published on: January 3, 2025

相关概念视频

Autophagic Cell Death

Autophagic Cell Death

Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...

Autophagy

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Delivery Pathways to the Lysosome

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...

Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin

Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin

Directly acting muscle relaxants like dantrolene and botulinum toxin (BoNT) have distinct mechanisms and applications. Dantrolene, a hydantoin derivative, acts on the ryanodine receptor (RYR1) in skeletal muscle cells. RYR1 are calcium channels present at the sarcoplasmic reticulum membrane. In response to excitation, they release calcium ions from the sarcoplasmic reticulum to the cytosol. Calcium promotes actin-myosin-mediated contraction of muscles.
The binding of dantrolene to the RYR1...

The Intrinsic Apoptotic Pathway

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...