二在肝细胞癌细胞中诱导亡:生化,病理和分子标记
Mahmoud I Khalil1,2, Alaa F Agamy2, Salma S Elshewemi3
1Department of Biological Sciences, Faculty of Science, Beirut Arab University, Lebanon.
Saudi journal of biological sciences
|July 24, 2023
概括
固醇 (PTE) 显著降低了肝细胞癌 (HCC) 细胞活力和殖民地形成. 这种天然化合物通过改变细胞结构和调节关键蛋白质来诱导亡,显示出作为抗癌剂的希望.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 在瘤学瘤学.
背景情况:
- 肝细胞癌 (HCC) 是全球癌症死亡的主要原因.
- 固醇 (PTE) 是蓝和葡萄中的植物化学物质,具有抗癌性.
研究的目的:
- 为了研究固醇 (PTE) 对HepG2肝细胞癌细胞的影响.
- 评估PTE对细胞活力,增殖,细胞亡和蛋白质表达的影响.
主要方法:
- 在PTE治疗的HepG2细胞中评估了细胞活力,殖民地形成,氧化应激标志物 (脂质过氧化,CAT,SOD),caspase-3活性和基因素释放.
- 利用光和传输电子显微镜进行细胞和超结构分析.
- 量化了mTOR,S6K1,p53和STAT3.3中的蛋白质水平.
主要成果:
- 以剂量取决的方式,PTE降低了HepG2细胞活力 (IC50 = 74 ± 6μM) 和殖民地形成.
- PTE改变了氧化应激标志物,增加了caspase-3活性和基因素释放,表明了亡.
- 超结构性变化包括细胞收缩,真空化,亡体和线粒体/ER变化. mTOR,S6K1和STAT3的下调,而p53的调节.
结论:
- 固醇对肝细胞癌细胞具有显著的抗癌潜力.
- 通过形态变化和关键细胞增殖蛋白的调制,PTE会诱导细胞亡.
- 作为HCC治疗的治疗剂,PTE显示出有前途.
关键词:
细胞灭亡 (apoptosis) 是一种死亡的过程.这是一种HepG2型肝炎.肝癌是一种肝癌.在P53中,P53是P53聚乙烯 (Pterostilbene) 是一种制剂.复星醇是一种复星.超结构的超结构在mTOROR中使用mTOR.更多相关视频
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