监测巨细胞的mRNA表达模式,以应对两种不同的益生菌菌株
Sang-Pil Choi1, Si-Won Park1, Seok-Jin Kang1
1Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea.
Food science of animal resources
|July 24, 2023
概括
这项研究表明,不同的益生菌菌株,Weissella cibaria WIKIM28和Latilactobacillus sakei WIKIM50,明显调节巨细胞两极分化. 益生菌可以根据它们对M1/M2巨细胞表型的影响来分类,影响免疫反应.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 分子生物学分子生物学
背景情况:
- 众所周知,益生菌会影响宿主免疫反应.
- 益生菌调节巨细胞活动的分子机制尚不完全理解.
- 巨细胞在先天性和适应性免疫中都发挥着关键作用,具有明显的M1 (前炎症) 和M2 (抗炎症/组织修复) 两极化状态.
研究的目的:
- 阐明益生菌介导的巨细胞调节的分子机制.
- 为了研究两个特定的益生菌菌株Weissella cibaria WIKIM28和Latilactobacillus sakei WIKIM50在巨分化上的差异作用.
- 为了确定关键的基因表达变化,与腹巨中的益生菌治疗相关.
主要方法:
- 腹膜巨细胞 (PMs) 用威塞拉西巴利亚WIKIM28或拉提亚菌 WIKIM50.50.治疗.
- 总RNA从受治疗和控制 (车辆治疗) 的PMs中分离出来.
- 进行基因芯片 (微阵列) 分析,以评估全基因组mRNA表达模式.
主要成果:
- 与载体对照组相比,在接受益生菌治疗的PM中观察到显著的基因表达差异.
- 与韦氏菌西巴利亚 WIKIM28相比,Latilactobacillus sakei WIKIM50治疗增加了M2巨分极标记的mRNA转录 (例如,anxa1, mafb, sepp1) 和减少了M1标记 (例如,hdac9, ptgs2, socs3).
- 与WIKIM28治疗的巨细胞相比,WIKIM50治疗的巨细胞中的瘤缩因子α和介质素-1αmRNA水平显著降低.
结论:
- 益生菌菌株在调节巨细胞极化方面表现出独特的能力.
- 拉提拉克托巴西勒斯 (Latilactobacillus sakei) WIKIM50促进了一个类似M2的巨细胞表型,而韦塞拉 (Weissella cibaria) WIKIM28可能会诱导不同的反应.
- 这些发现表明益生菌的分类基于它们对巨细胞两极分化的差异作用,影响免疫调节.
相关概念视频
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