在ATP7B中对Hepg2细胞中Lncrna-Mrna网络的识别,即在Knockout和铜积累时

相关实验视频

Updated: Jul 22, 2025

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

Yan Yan¹, Yin Xu¹, Lin Chen¹

¹Affiliated Hospital of the Institute of Neurology, Anhui University of Chinese Medicine, Hefei, 230061, P.R. China.

Iranian journal of public health

|July 24, 2023

概括

这项研究通过分析基因表达数据,探讨了肝细胞退化 (HLD) 的遗传变化. 关键发现表明,特定的lncRNA-mRNA相互作用对HLD发展至关重要,并表明潜在的治疗点.

科学领域:

遗传学是一个遗传学.
生物信息学是一种生物信息学.
分子生物学分子生物学

背景情况:

肝细胞变性 (HLD) 是一种遗传性疾病,与腺三酸铜运输β基因 (ATP7B) 的突变有关.
了解HLD的遗传基础对于开发有效的治疗方法至关重要.

研究的目的:

用生物信息学分析研究HLD中的遗传变化.
确定关键的lncRNAs,基因和涉及HLD病变发生的途径.

主要方法:

从尼泊尔 (2019) 下载并分析了GSE107323数据集.
使用Mfuzz集群分析在ATP7B淘汰和铜毒性后差异表达的lncRNAs (DElncRNAs) 和基因 (DEGs).
基于高共表达和通路分析构建了一个lncRNA-基因通路网络.

主要成果:

在ATP7B淘汰和铜毒性条件之间确定了51个重叠的DEG和687个重叠的DElncRNA.
该lncRNA-基因通路网络包括13个DElncRNA,10个DEG,并涉及Jak-STAT和Adipocytokine信号通路.
突出了八个下调基因 (EPO,IRS1,PPARGC1A) 和两个参与这些通路的上调基因,这些基因被像PCAT6和MALAT1这样的lncRNA所准.

结论:

不同表达的lncRNA-mRNA轴通过影响细胞增殖和炎症,对HLD病变产生显著贡献.
EPO,IRS1和PPARGC1A是HLD治疗的有希望的治疗点.

关键词:

氨酸三酸酶铜运输β 氨酸三酸酶铜生物信息学分析铜的积累铜的积累肝细胞退化症肝细胞退化症

更多相关视频

Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2

Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2

Published on: October 24, 2016

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Published on: October 23, 2018

相关概念视频

The Nucleolus

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...