没有平衡的热力学和线粒体蛋白质含量预测了人类骨肌肉在运动期间的胰岛素敏感性和燃料选择
Rocio Zapata Bustos1,2, Dawn K Coletta1,2,3, Jean-Philippe Galons4
1Division of Endocrinology, Department of Medicine, The University of Arizona, Tucson, AZ, United States.
Frontiers in physiology
|July 24, 2023
概括
骨架肌肉是一个骨肌肉.
科学领域:
- 运动生理学 运动生理学
- 代谢调节 代谢调节 代谢调节 代谢调节
- 骨肌肉生物学 骨肌肉生物学
背景情况:
- 肌肉中的分子变化传统上是针对胰岛素耐药性的研究.
- 肌肉的生理背景和能量状态,特别是ATP的热力学状态 (ΔGATP),也可能影响胰岛素敏感性.
- 这项研究调查了肌肉能量状态在胰岛素敏感性和燃料选择中的作用.
研究的目的:
- 要确定骨肌的热力学状态是否解释胰岛素敏感性和燃料选择.
- 评估ATP的热力学状态 (ΔGATP) 和胰岛素刺激过程之间的关系.
- 探索线粒体功能对燃料氧化的独立贡献.
主要方法:
- 在16名志愿者中使用P-MRS,葡萄糖,运动测试和肌肉活检.
- 测量胰岛素敏感性,热力学变量,线粒体蛋白质含量和有氧容量 (VO2max).
- 采用线性电路模型和多重阶段回归分析.
主要成果:
- VO2max和ΔGATP与氧化酸化的斜率解释了胰岛素敏感性变异的53%.
- 斜率表示线粒体的功能内容 (导电).
- 线粒体蛋白质含量独立预测了运动期间的微分脂肪氧化 (R2 = 0.55).
结论:
- 更高的线粒体功能含量支持更大的ΔGATP,可能增强胰岛素刺激的过程.
- 线粒体蛋白质含量直接影响轻度运动期间的微分脂肪氧化.
- 肌肉的能量状态,特别是线粒体功能,对于代谢调节和胰岛素敏感性至关重要.
更多相关视频
09:40Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle
Published on: January 19, 2017
11.8K
07:18Author Spotlight: New Insights into PBMC Mitochondrial Responses Using Fluorespirometry
Published on: May 24, 2024
1.1K
相关概念视频
01:37Energy to Drive Translocation
2.1K
Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...
Generally, polypeptides are unfolded by two distinct...
2.1K
01:19Translocation of Proteins into the Mitochondria
3.2K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.2K