甲氨酸氧化有选择地增强T细胞对黑色素瘤抗原的反应性
Gabriela N Chiriţoiu1, Cristian V A Munteanu2, Teodor A Şulea2
1Department of Molecular Cell Biology, Institute of Biochemistry, Splaiul Independenței 296, 060031 Bucharest, Romania.
iScience
|July 24, 2023
概括
黑色素瘤中的甲酸氧化增强了它们的抗原性,可能增加T细胞的反应. 这种氧化在体内自然发生,这表明它在黑色素瘤进展和免疫监测中发挥了作用.
科学领域:
- 免疫学 免疫学 免疫学
- 生物化学 生物化学
- 癌症研究 癌症研究
背景情况:
- 对侧链修饰如何影响免疫识别的理解有限.
- 甲氨酸氧化是一种翻译后的修饰,其免疫学后果在很大程度上尚未被探索.
研究的目的:
- 研究甲酸氧化对黑色素瘤免疫主导 (YMD) 抗原性的影响.
- 探索氧化黑色素瘤的体内发生和免疫学影响.
主要方法:
- CD8+ T 细胞激活试验测量抗原性.
- 在HLA-A*02:01/YMD/TCR复合体上的自由能量计算.
- 液体染色学-并联质谱学 (LC-MS/MS) 用于体内鉴定.
主要成果:
- 与原生YMD相比,氧化YMD具有更高的抗原性.
- 计算分析表明,氧化时结合自由能量减少,原子秩序增加.
- 在黑色素瘤细胞表面检测到氧化的YMD形式.
结论:
- 甲氨酸氧化可以产生具有增强抗原性的改变联体.
- 这种氧化过程在黑色素瘤进展期间发生在体内.
- 自然原始的高亲和度CD8+ T细胞可能来自氧化抗原,影响免疫监测.
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