相关实验视频
Updated: Jul 22, 2025
09:47
Analysis of Histone Antibody Specificity with Peptide Microarrays
Published on: August 1, 2017
40.4K
通过H3K9bhb抗体对基因组修饰的非特异性识别
Takeshi Tsusaka1, Juan A Oses-Prieto2, Christina Lee3
1Department of Physiology, University of California, San Francisco, San Francisco, CA 94158, USA.
iScience
|July 24, 2023
概括
体,如β-基酸盐 (BHB),信号细胞. 虽然 lysine beta-hydroxybutyrylation (Kbhb) 是一个关键的修饰,但常见的H3K9bhb抗体可能会检测出其他变化,包括乙化.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 体,如β-基酸盐 (BHB),在低葡萄糖可用性期间作为替代能源.
- BHB越来越多地被认为是超出其代谢功能的信号分子.
- lysine beta-hydroxybutyrylation (Kbhb) 是一种新的翻译后修饰,其中 BHB 附着在 lysine 残留物上,影响蛋白质功能.
研究的目的:
- 为了研究氨酸β-基丁化 (Kbhb) 作为信号机制的作用.
- 评估用于检测Kbhb的抗体的特异性,特别是在基因质上.
- 了解代谢状态,蛋白质修饰和基因表达之间的联系.
主要方法:
- lysine beta-hydroxybutyrylation (Kbhb) 作为一个翻译后修改的表征.
- 使用各种生化分析评估抗H3K9bhb抗体的特异性.
- 分析与其他氨酸修饰物 (如乙化) 的潜在交叉反应.
主要成果:
- lysine beta-hydroxybutyrylation (Kbhb) 是一种在不同细胞区中发现的代谢敏感的修饰.
- 广泛使用的对素H3 lysine 9β-hydroxybutyrylation (H3K9bhb) 的抗体具有交叉反应性.
- 这种交叉反应性包括识别其他影响特异性的修饰,可能包括乙化.
结论:
- lysine beta-hydroxybutyrylation (Kbhb) 是一个重要的修改,将代谢状态与细胞生理学联系起来.
- 由于潜在的交叉反应性,建议在解释从H3K9bhb抗体获得的数据时谨慎.
- 对抗体的进一步验证对于准确评估Kbhb在表观遗传调节和基因表达中的作用至关重要.
相关概念视频
02:32Histone Modification
13.4K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.4K
02:25Spreading of Chromatin Modifications
8.3K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
8.3K
02:30Histone Variants at the Centromere
4.4K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.4K
01:12The Nucleosome Core Particle
946
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
946
02:36Chromatin Immunoprecipitation- ChIP
11.2K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
11.2K