一种新型大豆粉化-复合物的开发,表征和活体吸收能力
Rongxin Wang1, Meijun Ye1, Suyin Zhu1
1School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou, China.
Frontiers in nutrition
|July 24, 2023
概括
豆粉化物- (SMHs-Zn) 复合物有效地增强小鼠的吸收,类似于更昂贵的合成-复合物. 这表明的生物可用性可能更多地取决于氨基酸成分而不是结合能力.
科学领域:
- 营养生物化学 营养生物化学
- 食品科学与技术 食品科学与技术
背景情况:
- 是一种必不可少的微量元素,对人类健康至关重要.
- 豆蛋白水解剂产生了一种具有高容量的新型Zn结合 (PP).
- 豆粉水解剂 (SMHs) -Zn复合物为PP-Zn提供了一个成本效益高的替代品.
研究的目的:
- 在小鼠模型中研究SMHs-Zn复合物的有效性,以提高的吸收.
- 为了比较SMHs-Zn与合成 (PP) -Zn复合物的对生物利用性的影响.
- 阐明SMHs-Zn复合物的结合机制和安全性.
主要方法:
- 豆粉的酶解以制备SMH; PP的合成.
- 频谱分析以确定结合机制.
- 用于细胞毒性评估的CCK-8试验.
- 在小鼠中评估吸收标记 (Zn,蛋白质,白蛋白,酶活性,PepT1/ZnT1mRNA).
主要成果:
- 通过氧和氨基与SMH结合,通过疏水/静电相互作用稳定.
- SMHs-Zn具有良好的溶解性,小颗粒大小,并促进Caco-2细胞生长.
- 在小鼠中,SMHs-Zn和PP-Zn都增加了含量和与相关的酶活性.
- 在小肠中,SMHs-Zn显示了比PP-Zn更高的PepT1和ZnT1mRNA表达.
结论:
- 尽管结合能力较低,但SMHs-Zn复合体显示出与小鼠中的PP-Zn相似的吸收增强.
- -复合物的生物可用性可能会受到氨基酸成分的影响,而不仅仅是Zn结合能力.
- SMHs-Zn代表了一种有希望的,具有成本效益的策略,用于提高的生物可用性.
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