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Updated: Jul 22, 2025

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使用Agrobacterium tumefaciens在CavCosmos sulphureus上的高效的花浸泡转化方法

Aziz Purwantoro^1,2, Muhammad Burhanuddin Irsyadi², Widhi Dyah Sawitri^1,2

¹Department of Agronomy, Faculty of Agriculture, Universitas Gadjah Mada, Rd. Flora, Bulaksumur, Sleman 55281, Yogyakarta, Indonesia.

Saudi journal of biological sciences

|July 24, 2023

概括

为黄色宇宙 (Cosmos sulphureus) 开发了一种高效的花浸泡转换方法,实现了12.78%的转换效率. 这种方法优化了Agrobacterium培养密度,表面活性剂度和浸泡时间,用于基因工程应用.

科学领域:

植物生物技术植物生物技术
基因工程是一种基因工程.
分子生物学分子生物学

背景情况:

黄色宇宙 (Cosmos sulphureus) 是基因工程的一个合适的模型.
农细菌介导的转化是植物基因工程中的一个关键技术.
之前没有报告过黄色宇宙的花浸泡转变.

研究的目的:

为黄色宇宙开发一种高效有效的花浸泡转化方法.
为了优化Agrobacterium介导的花浸泡转换的关键参数.

主要方法:

优化了使用花 (5-7毫米) 的花浸泡转变.
不同的Agrobacterium光学密度 (OD600),表面活性剂度 (Silwet L-77) 和浸泡时间.
通过PCR确认了基因整合 (neomycin phosphotransferase II,绿色光蛋白).

主要成果:

最佳条件:Agrobacterium OD600 = 0.8,5%的糖糖,0.1%的Silwet L-77,30秒浸泡时间.
实现了12.78 ± 1.53%的转换效率.
转基因植物表现出对卡纳米辛的耐药性和绿色光.

结论:

开发的花浸泡方法是有效的黄色宇宙基因工程.
这项研究为转换黄色宇宙提供了一种有价值的新方法.
这些发现有助于推进植物遗传工程技术.

关键词:

花浸泡花浸泡水.在OD OD中,OD是OD.一个叫Silwet L-77的公司.表面活性剂是一种表面活性剂.转基因的转基因黄色的宇宙是一个黄色的宇宙.

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