相关实验视频

Updated: Jul 22, 2025

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids

Published on: August 9, 2024

在酶膜反应堆中复杂的织废水的排毒和脱色:批量和连续研究

Manju Dahiya¹, Dar Tafazul Islam¹, Preeti Srivastava¹

¹Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi, India.

Frontiers in microbiology

|July 24, 2023

概括

这项研究表明,一种工程化的乳酶酶 (LCC1-62) 能够有效地去色和排毒织染料废水. 这种基于生物的技术显示出大规模,环保的工业废水处理的前景.

科学领域:

生物技术和环境科学生物技术和环境科学
酶工程是什么? 酶工程是什么?
废水处理废水处理

背景情况:

织行业的废水对水体构成重大污染挑战.
生物基技术为处理染料污染提供了可持续的解决方案.
乳酶酶是强大的氧化还原酶,具有染料脱色的潜力.

研究的目的:

评估来自*Cyathus bulleri*的工程乳酸酶变体 (LCC1-62),用于织废水脱色和排毒.
在批量和连续模式下评估LCC1-62在射流膜反应堆中的性能.
确定使用这种酶用于大规模工业废水处理的可行性.

主要方法:

在重组*Pichia pastoris*中表达工程酶LCC1-62的表达.
在批量和连续射线流膜反应器中测试部分净化乳酸盐对真实织废水的脱色.
在各种操作条件下 (HRT,酶剂量) 评估脱色效率,排毒,酶活性和膜污染.

主要成果:

拉卡斯LCC1-62实现了真实织废水的高脱色 (~60-100%) 和排毒 (~96%).
在膜反应器中以6小时的液压保留时间 (HRT) 连续运行,产生了~95%的脱色和96%的变异性降低.
在延长反应堆运行期间观察到最小的膜污染 (<10%),表明系统稳定性.

结论:

工程制造的乳酶LCC1-62在脱色和排毒织染料废水方面非常有效.
使用LCC1-62的光流膜反应器系统证明了大规模工业废水处理的可行性.
这种基于生物的方法为传统的废水处理方法提供了可持续和高效的替代方案.

关键词:

连续处理连续处理工程拉卡赛斯 (laccase) 是一个工程拉卡赛斯.酶膜反应器中的酶膜反应器.真正的织废水是真实的再组合的乳糖酶.

更多相关视频

Bioreactor Assembly for Continuous Culture of Complex Fecal Communities

Bioreactor Assembly for Continuous Culture of Complex Fecal Communities

Published on: April 25, 2025

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Published on: October 15, 2015

相关概念视频

Detergent Purification of Membrane Proteins

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...

Dialysis

Dialysis

Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...