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A S Jaramillo-Jaramillo¹, T J D Coulson¹, C Hofacre²

¹MicroSintesis Inc., Victoria, P.E.I. Canada.

Avian pathology : journal of the W.V.P.A

|July 24, 2023

概括

来自Enterococcus faecium的无细胞消耗介质减少了 brojlers中的死性肠炎死亡率. 生物活性干扰了Clostridium perfringens的质量感应系统,在不改变肠道微生物群的情况下降低了毒性.

科学领域:

动物科学动物科学
微生物学微生物学
兽医医学兽医医学兽医医学

背景情况:

结核性肠炎 (NE) 对家禽产业产生重大影响,由Clostridium perfringens NetB毒素引起.
细菌的毒性,包括NetB的产生,是通过定数感应 (QS) 系统来调节的.
NE导致生长率下降和肉的料转换受损.

研究的目的:

评估Enterococcus faecium无细胞消耗介质 (CFSM) 在减轻 brojlers中NE的疗效.
调查CFSM对C. perfringens毒性和阴微生物群的影响.
确定CFSM影响C. perfringens基因表达和溶血活性的机制.

主要方法:

在受控研究中,肉受到C. perfringens的挑战,并接受E. faecium CFSM的治疗.
甲基因组测序分析了阴微生物组的组成.
在体外测试中评估了C. perfringens的生长和血液溶解活性.
定量PCR测量了QS控制的毒性基因 (netB,plc,pfoA) 的表达.

主要成果:

CFSM显著降低了肉中NE相关的死亡率.
没有观察到对真肠微生物群体或C. perfringens在体外生长的显著影响.
CFSM显著抑制了QS控制的毒性基因的表达,并降低了溶血活性.
来自CFSM的纯化生物活性可以减少细菌血液溶解.

结论:

在E. faecium CFSM中的生物活性有效地降低了 brojlers的NE死亡率.
该机制涉及干扰C. perfringens的质量感应系统,导致病毒性降低.
CFSM治疗不会对受到挑战的 brojlers 的肠道微生物组产生负面影响.

关键词:

克洛斯特里 (Clostridium perfringensens) 是一种有毒的植物.网B 网B 在线 brojler 肉肉肉是一个血液溶解血液溶解微生物组是一个微生物组.死亡性肠炎 (necrotic enteritis) 是一个疾病.议员数量检测是指对议员数量进行检测.病毒性基因表达病毒性基因表达

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