相关实验视频

Updated: Jul 22, 2025

Author Spotlight: Advanced Integrated Model for Sepsis-Induced Myopathy and Single-Cell Metabolic Analysis

Author Spotlight: Advanced Integrated Model for Sepsis-Induced Myopathy and Single-Cell Metabolic Analysis

Published on: June 14, 2024

微RNA-140不参与败血症引起的肌肉缩

Jaehoon Shin¹, Shigeru Miyaki², Hiroshi Asahara³

¹Laboratory of Muscle Biology, Faculty of Sport Sciences, Waseda University, Saitama, Japan.

American journal of physiology. Cell physiology

|July 24, 2023

概括

微RNA-140 (miR-140) 不影响脂聚糖 (LPS) 诱导的骨肌肉缩. 在小鼠中删除miR-140并没有改变LPS暴露后的肌肉消耗或蛋白质分解标志物.

科学领域:

生物化学生物化学
生理学生理学生理学
分子生物学分子生物学

背景情况:

败血症会引发严重的炎症反应,往往导致骨肌肉缩.
以前的研究表明,microRNA-140 (miR-140) 在缓解脂聚糖 (LPS) 诱导的肌肉衰竭方面发挥着作用.

研究的目的:

通过使用遗传淘汰模式,研究miR-140在LPS诱导的骨肌缩中的作用.
分析miR-140删除对慢和快肌群的影响.

主要方法:

使用miR-140淘汰赛小鼠和野生类型 (WT) 的 littermates.
用单剂量脂多糖 (LPS) 诱导类似败血症的情况.
评估肌肉重量,纤维横截面积,以及关键蛋白质分解标记物的表达 (MuRF1,MAFbx/atrogin-1).

主要成果:

在WT和miR-140小鼠中,LPS注射诱导了显著的肌肉缩.
在基因型之间没有观察到肌肉缩或蛋白质分解标志物表达的显著差异.
在LPS给药后,在WT和miR-140小鼠中,ubiquitinated蛋白质水平是可比的.

结论:

缺少miR-140不会加剧或保护LPS诱导的骨肌肉缩.
在这个模型中,miR-140不是在LPS暴露后肌肉消耗的发展的关键因素.
这项研究提供了关于miR-140操纵的LPS诱导肌肉消耗中缓慢抽肌肉反应的新见解.

关键词:

灭症 (apoptosis) 是一种死亡的过程.肌肉消耗肌肉消耗肌肉消耗没有编码的RNA.蛋白质溶解过程中的蛋白质溶解.

更多相关视频

Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents

Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents

Published on: August 10, 2018

Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice

Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice

Published on: February 9, 2014

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Formation of Muscle Fibers from Myoblasts

Formation of Muscle Fibers from Myoblasts

De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...