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Updated: Sep 12, 2026

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植物组织的固定和激光捕获微解剖,用于RNA提取和RNASeq库准备

Suchitra Chavan1, Elise Schnabel1, Christopher Saski2

  • 1Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina.

Current protocols
|July 24, 2023
PubMed
概括

研究人员开发了激光捕获微解剖 (LCM) 的协议,以隔离单个植物细胞进行转录组分析. 这种方法产生高质量的RNA用于RNA测序,使得在特定细胞类型中进行基因表达研究.

关键词:
提取RNA 提取RNA的方法在RNASeq中使用.不同的表达方式,不同的表达方式.激光捕捉微切割微切割植物组织的固定固定.

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相关概念视频

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...