假定NAD (((P) 结合的罗斯曼折叠蛋白涉及酸盐诱导的过氧化酶活性和氧化酶表达Physcomitrium patens
Eeva M Marttinen1, Eva L Decker2, Petra Heinonen1
1Department of Agricultural Sciences, PO Box 27, FI-00014 University of Helsinki, Finland.
Molecular plant-microbe interactions : MPMI
|July 24, 2023
概括
在Physcomitrium patens中,一种新的罗斯曼折叠蛋白对氧化爆发防御反应至关重要. 淘汰植物在奇托治疗后显示过氧化酶活性降低和氧化酶基因表达降低.
科学领域:
- 植物科学 植物科学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 氧化突发,即快速反应性氧物种的产生,是植物对病原体的关键防御.
- 素是一种真菌诱导剂,在Physcomitrium patens中触发氧化突发,由过氧化酶Prx34.4介导.
研究的目的:
- 为了确定参与P. patens.中素诱导的氧化爆发途径的基因.
- 研究一个NAD (((P) 结合的罗斯曼折叠蛋白在植物防御反应中的作用.
主要方法:
- 查P. patens突变集合,以检测在基托治疗后的过氧化酶活性降低.
- 产生和分析罗斯曼折叠蛋白敲击 (KO) 线.
- 评估细胞外过氧化酶活性和防御基因表达 (LOX,Prx34,NADPH氧化酶,替代氧化酶).
主要成果:
- 罗斯曼折叠KO线表现出显著较低的细胞外过氧化酶活性与野生类型 (WT) 植物相比,在基托桑治疗后.
- 氧化酶 (LOX) 基因表达在罗斯曼折 KO 线上在奇托应用之前和之后都显著减少.
- 罗斯曼折叠蛋白似乎对基底LOX基因表达和氧化爆发信号通路至关重要.
结论:
- 结合NAD(P) 的罗斯曼折叠蛋白是P. patens.中素诱导的氧化突发信号通路的关键组成部分.
- 这种蛋白质影响氧化突发和氧化酶基因的基本表达,突出其在植物免疫中的调节作用.
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