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相关概念视频

Reporter Genes02:11

Reporter Genes

11.6K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Riboswitches01:56

Riboswitches

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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相关实验视频

Updated: Jul 22, 2025

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
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Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

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基因编码的基于RNA的传感器,配有Pepper原性胺体.

Zhenyin Chen1,2,3, Wei Chen1,4, Zhayila Reheman1,5

  • 1Beijing Institute of Life Sciences, Chinese Academy of Sciences, Beijing 100101, China.

Nucleic acids research
|July 24, 2023
PubMed
概括
此摘要是机器生成的。

基于Pepper的新RNA传感器,这是一种没有RG4动机的基RNA,能够对活细胞中的细胞内分子和通路进行强大的成像. 这些传感器为各种目标提供高激活性和选择性.

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相关实验视频

Last Updated: Jul 22, 2025

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
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Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

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G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay
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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
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科学领域:

  • 分子生物学分子生物学
  • 生物化学 生物化学
  • 细胞生物学 细胞生物学

背景情况:

  • 细胞内分子传感对于理解生理功能至关重要.
  • 传统的光蛋白传感器存在,但基于RNA的传感器提供了独特的优势.
  • 现有的RNA传感器通常含有RNA G-四重复 (RG4) 基因,这可能会限制它们在体内的有效性.

研究的目的:

  • 利用Pepper开发基于RNA的新型传感器,Pepper是一种缺乏RG4图案的基RNA.
  • 设计和验证各种细胞内标的传感器,包括代谢物,蛋白质和金属离子.
  • 展示这些传感器在监测细胞通路和评估活细胞中药物疗效方面的应用.

主要方法:

  • 工程基于RNA的传感器使用Pepper化RNA平台.
  • 在体外和体内验证传感器性能,包括激活和选择性.
  • 开发一种S-adenosylmethionine (SAM) 的比度传感器,以评估药物抑制.

主要成果:

  • 成功开发了基于胡的RNA传感器,用于各种目标,在体外和活细胞中发挥作用.
  • 工程传感器的高激活,选择性,通用性和强度得到了证明.
  • 成功监测了S-adenosylmethionine (SAM) 生物合成,氨酸腺转移酶 (MATase) 活性和基因表达的空间时间.
  • 使用比度SAM传感器量化了一种MATase抑制剂的抑制功效.

结论:

  • 基于胡的RNA传感器为成像各种细胞目标提供了一个多功能和强大的平台.
  • 这些传感器可以实时监测活细胞中的分子信号通路和药物反应.
  • 开发的传感器显著提高了细胞内分子检测和途径分析的能力.