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相关概念视频

RNA Structure01:23

RNA Structure

71.6K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
71.6K
Nucleic Acid Structure01:25

Nucleic Acid Structure

6.2K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
6.2K
RNA Stability01:53

RNA Stability

33.7K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.7K
Nucleic acids02:43

Nucleic acids

163.7K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
163.7K
Protein Organization01:13

Protein Organization

138.6K
Overview
138.6K
RNA-seq03:21

RNA-seq

10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K

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相关实验视频

Updated: Jul 22, 2025

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

31.6K

使用RNA结构进行RNA二次结构分析.

Sara E Ali1, Abhinav Mittal1, David H Mathews1

  • 1Department of Biochemistry & Biophysics and Center for RNA Biology, University of Rochester Medical Center, Rochester, New York.

Current protocols
|July 24, 2023
PubMed
概括
此摘要是机器生成的。

RNA结构软件提供了用户友好的工具,用于预测RNA二次结构和寡核酸结合点. 在多个操作系统中提供了Web,图形和命令行接口的协议.

关键词:
预测RNA的二次结构尽量减少自由能源的使用.有关寡核酸结合的亲缘关系.热力学 热力学 热力学

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

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Analyzing and Building Nucleic Acid Structures with 3DNA
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Analyzing and Building Nucleic Acid Structures with 3DNA

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相关实验视频

Last Updated: Jul 22, 2025

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

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Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

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科学领域:

  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学

背景情况:

  • 预测RNA二次结构对于理解RNA功能至关重要.
  • 分析RNA-蛋白和RNA-RNA相互作用需要准确的结构预测.
  • 现有的工具可能缺乏用户友好性或全面的功能.

研究的目的:

  • 提供使用RNA结构进行RNA二次结构预测的可访问协议.
  • 详细介绍识别结构RNA上的高亲和度寡核酸结合点的方法.
  • 通过Web,GUI和命令行接口提供多功能RNA结构分析选项.

主要方法:

  • 利用RNA结构Web服务器进行快速的二级结构预测.
  • 使用RNA结构图形用户界面 (GUI) 进行详细的结构和基对概率分析.
  • 使用RNAstructure命令行接口进行自动化的二次结构预测.
  • 在RNA结构GUI中应用OligoWalk工具来预测结合亲和关系.

主要成果:

  • 证明了RNA结构在不同平台 (web,GUI,命令行) 上预测RNA二级结构的实用性.
  • 成功概述了用于预测基数对概率的协议,提高了结构准确性.
  • 提供了一种方法来识别潜在的寡核酸结合点及其与RNA标的亲缘关系.

结论:

  • RNAstructure是一个多功能和用户友好的软件套件,用于全面的RNA二次结构分析.
  • 提供的协议使研究人员能够有效地预测RNA结构和结合点.
  • 多个接口的可用性确保了对各种研究需求的广泛可用性.