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通过三维光场照明进行光学单像素体积成像

Yifan Liu¹, Panpan Yu¹, Yijing Wu¹

¹Department of Optics and Optical Engineering, University of Science and Technology of China, Hefei 230026, China.

Proceedings of the National Academy of Sciences of the United States of America

|July 24, 2023

概括

研究人员开发了用于高分辨率3D成像的3D光场照明单像素显微镜 (3D-LFI-SPM). 这种技术实现了对微观物体的近衍射极限分辨率,推动了生物医学研究.

科学领域:

光学和光子学在光学和光子学.
生物医学成像技术生物医学成像技术
显微镜的使用方法

背景情况:

三维单像素成像 (3D SPI) 对生物医学研究和光学传感非常有价值.
目前使用飞行时间或立体视觉的3D SPI方法仅限于用于表面映射的毫米级深度分辨率.
需要具有更高分辨率的3D成像技术,特别是用于显微镜应用.

研究的目的:

介绍和演示一种新的3D SPI技术,即3D光场照明单像素显微镜 (3D-LFI-SPM).
为了实现显微物体的体积成像,使用接近衍射极限的3D光学分辨率.
为了使生物样本中光学吸收对比度的无标签3D可视化.

主要方法:

3D-LFI-SPM在光学上采样了3D里埃光谱.
将3D结构光场照明与单元强度检测相结合.
为体积成像建造了一个原型系统.

主要成果:

3D-LFI-SPM原型实现了大约390 × 390 × 3,800 μm3.3的成像体积.
证明横向分辨率为2.7微米,轴向分辨率优于37微米.
在单个藻类细胞中成功可视化无标签的光学吸收对比 in vivo.

结论:

3D-LFI-SPM可实现显微结构的高分辨率体积成像.
该技术比现有的3D SPI方法提供了显著的改进.
这种方法对3D SPI应用具有广泛的潜力,包括先进的生物医学功能成像.

关键词:

3D光场照明照明 3D光场照明一个像素的成像.容量成像成像容量成像成像

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