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微RNA作为犬类cryptorchidism的表观遗传调节者

Giulia Pizzi¹, Debora Groppetti¹, Eleonora Brambilla¹

¹Università degli Studi di Milano, Lodi 26900, Italy.

Research in veterinary science

|July 24, 2023

概括

微RNAs (miRNAs) 在犬类密码和受半瘤影响的丸中失调. 这项研究研究了保留和癌症犬丸中的miRNA表达,揭示了改变的水平对于理解这些条件至关重要.

科学领域:

兽医医学兽医医学兽医医学
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

阴囊症,丸下降的失败,是一种常见的狗疾病,影响生育能力,并可能导致瘤.
微RNAs (miRNAs) 是关键的表观遗传调节剂,其失调与精子生成障碍和丸瘤有关.
了解miRNA在犬丸疾病中的作用对于诊断和治疗至关重要.

研究的目的:

为了研究特定的miRNAs的表达形式素固定,巴拉芬嵌入 (FFPE) 狗保留的丸和受精子瘤影响的丸.
将miRNA表达数据与它们的潜在目标基因集成.
探索miRNAs在犬类密码性瘤和丸性瘤的发病过程中的作用.

主要方法:

来自30只狗的40个丸FFPE标本的分析,包括阴囊,保留,瘤 (精髓瘤) 和对照丸.
使用定量逆转录聚合酶链反应 (RT-qPCR) 来量化miR-302c-3p,miR-302a-3p和miR-371-3p的表达水平.
基因实体学分析以确定受研究miRNAs调节的途径.

主要成果:

miR-302a-3p和miR-371a-3p只能在对照试验中得到量化.
miR-302c-3p的表达在对照丸中最高,在保留丸和受半瘤影响的丸中降低.
基因实体学分析表明,这些miRNAs参与雌激素和甲状腺激素信号通路.

结论:

与正常对照对象相比,MicroRNA表达在犬类密码虫和受精子受影响的丸中显著改变.
这些发现证实了miRNAs在犬密码症和相关的丸疾病的发展和进展中起着关键作用.
失调的miRNAs可以作为犬丸病理的潜在生物标志物.

关键词:

密码主义是什么?密码主义是什么?狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗这里是Seminoma,Seminoma和Seminoma.这是一个微型RNA.

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