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相关概念视频

Signal Sequences and Sorting Receptors01:41

Signal Sequences and Sorting Receptors

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Signal sequences are short amino acid sequences that guide newly synthesized proteins to their proper location within the cell. Classical signal sequences are fifteen to sixty amino acids long and present at the N-terminus of a polypeptide chain. Each signal sequence has a conserved segment of basic residues towards their N terminus, a hydrophobic core, and a C-terminus rich in polar residues. The C-terminus also contains a signal cleavage site and features a -3 -1 sequence motif. The -3-1...
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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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Bacterial Translocation and Protein Secretion01:26

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Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
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Integral membrane proteins are proteins adhered to the lipid bilayer of a cell organelle or membrane. They can be of two types: transmembrane integral proteins that span the lipid bilayer and monotopic proteins that are attached to either side of the membrane but do not pass through it.
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Updated: Jul 22, 2025

Engineering Cell-permeable Protein
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用于哺乳动物载体工程的蛋白特异信号.

Pamela O'Neill1, Rajesh K Mistry2, Adam J Brown1,3

  • 1Department of Chemical and Biological Engineering, University of Sheffield, Mappin Street, Sheffield S1 3JD, U.K.

ACS synthetic biology
|July 24, 2023
PubMed
概括
此摘要是机器生成的。

研究人员为哺乳动物细胞工厂开发了一种新的信号工具包,以促进生物制药生产. 该系统使用机器学习和计算设计来优化蛋白质表达,显著增加各种治疗蛋白质的产量.

关键词:
哺乳动物哺乳动物重组蛋白质蛋白质的重组蛋白质是什么?信号是一种信号.转移转移是指转移的转移.

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科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 哺乳动物细胞工厂对于生物制药生产至关重要,需要优化基因组件来控制蛋白质合成.
  • 哺乳动物系统中存在有限数量的特征信号,阻碍了高效的重组蛋白表达.

研究的目的:

  • 创建和验证一个信号素元素工具包,以增强中国仓鼠卵巢 (CHO) 细胞中的生物制药生产.
  • 应用机器学习和计算方法来预测信号的功能,并优化蛋白质转移到内 плазма网膜 (ER).

主要方法:

  • 生物信息学主导和合成设计方法被用来创建一个新的信号工具包.
  • 开发了机器学习模型,以预测信号在驱动单链蛋白的ER转位中的有效性.
  • 基于规则的in silico和in vitro设计测试被结合起来,以确定像单克隆抗体这样的复杂多链蛋白的最佳信号.

主要成果:

  • 与标准方法相比,开发的信号工具包和设计策略显著提高了至少1.8倍的蛋白质生产标位.
  • 该研究展示了机器学习的首次成功应用,用于预测哺乳动物细胞中信号的性能.
  • 优化的载体设计被生成,从而提高了各种蛋白质产品的产量,包括具有挑战性的单克隆抗体.

结论:

  • 扩展的特征信号的工具包,加上精简的计算和实验测试,可以有效地重新设计表达向量.
  • 这种方法最大限度地提高了哺乳动物细胞工厂中简单和复杂的生物制药产品的蛋白位.
  • 这些发现为加速生物制剂的开发和生产提供了一个强大的平台.