在角膜上皮细胞脱bridement之后,FoxC1激活四肢上皮干细胞
Wending Yang1, Sun Kyong Lee2, Ordan J Lehmann3
1Departments of Dermatology, Feinberg School of Medicine, Northwestern University, Chicago, IL, 60611, USA.
Experimental eye research
|July 24, 2023
概括
在Pax6+/-小鼠中过度表达FOXC1可增强肢体干细胞功能,促进干细胞增殖和干细胞标志物表达,特别是在角膜损伤后.
科学领域:
- 眼科医生 眼科 眼科
- 干细胞生物学 干细胞生物学
- 发展生物学 发展生物学
背景情况:
- 皮上皮干细胞 (LESCs) 保持角膜健康,并对损伤做出反应.
- 帕克斯6缺乏导致肢体干细胞缺乏症 (LSCD) 现型,包括角膜血管化.
- 福克斯C1影响干细胞维护和角膜无血管性,但其在LESC中的作用尚不清楚.
研究的目的:
- 为了研究FoxC1在肢体上皮质干细胞平衡中的作用.
- 为了确定FoxC1是否可以在Pax6+/-小鼠中拯救LSCD表型.
主要方法:
- 腺相关病毒 (AAV) 载体用于将人类FOXC1局部输送到Pax6+/-小鼠四肢表皮.
- 对差异化标志物 (PAI-2,Krt12) 和扩散标志物的分析 (BrdU,Ki67).
- 估计的LESC标志物的评估 (N-cadherin,Lrig1) 表达.
主要成果:
- 仅FOXC1过度表达在正常条件下对分化和增殖的影响很小.
- 过度表达FOXC1显著增加了N-cadherin和Lrig1的表达,表明增强了干细胞的特性.
- 在患有角膜损伤的Pax6+/-小鼠中,FOXC1过度表达促进了LESC的扩散.
结论:
- FOXC1在调节LESC的行为和干度方面发挥着作用.
- 由AAV介导的FOXC1输送通过增强LESC功能,特别是在Pax6缺陷的背景下,为LSCD显示了潜在的治疗益处.
相关概念视频
01:19Clinical Applications of Epidermal Stem Cells
2.7K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
2.7K
01:12Renewal of Skin Epidermal Stem Cells
2.5K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.5K
01:23Tissue Renewal without Stem Cells
1.7K
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
However, failure of such a system...
1.7K