一个三方微生物共同培养系统,用于各种植物烯胺的新生合成
Sierra M Brooks1, Celeste Marsan1, Kevin B Reed1
1McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.
Nature communications
|July 24, 2023
概括
这项研究引入了一种新的微生物共同培养,用于从糖中生产新型基,克服了以前的制造限制. 这种工程系统能够可持续地生产有价值的植物化合物,如欧原醇,氧沙维醇和沙维醇.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 生物技术是生物技术.
背景情况:
- 植物衍生的烯,特别是烯,具有重要的工业价值.
- 目前的制造方法面临着由于低,季节性产量和复杂的开采工艺的挑战.
- 异质生物合成为可持续生产提供了一个有希望的替代方案.
研究的目的:
- 开发一种微生物共同培养系统,从简单糖中重新生产基.
- 克服微生物生物合成中途杂交和中间毒性的局限性.
- 建立一个生产各种植物二次代谢物的多功能平台.
主要方法:
- 三方微生物共同培养的设计和实施.
- 在路径工程中利用模块化和酶乱交.
- 与工程生物材料 (ELM) 平台集成,用于分布式制造.
主要成果:
- 通过微生物共同培养,首次证明了从糖中产生新型烯的生产.
- 成功生产了欧原醇,氧沙维醇和沙维醇.
- 展示了与可重复使用,分布式制造的ELM平台的兼容性.
结论:
- 三方微生物共同培养有效克服了烯生物合成的局限性.
- 该平台为模块化微生物生产各种植物二次代谢物的基础.
- 工程生物材料提供了一个可持续和分散的制造方法.
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