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Mn-MOF催化多位点原子转移激素聚合电化学传感miRNA-21的电化学感应

Jiao Wang¹, Jingliang Liu², Lianzhi Li³

¹School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu, 210094, People's Republic of China.

Mikrochimica acta

|July 24, 2023

概括

一种新的绿色电化学生物传感器利用金属有机框架 (MOF) 催化剂进行超敏感检测miRNA-21. 这种方法在血清样本中提供了早期临床诊断的潜力.

科学领域:

电化学电化学电化学
材料科学材料科学材料科学
生物技术是生物技术.

背景情况:

微RNA-21 (miRNA-21) 是各种疾病的生物标志物.
传统的电化学生物传感器通常依赖于有毒的催化剂.
开发绿色和敏感的检测方法对于早期疾病诊断至关重要.

研究的目的:

开发一种绿色电化学生物传感器,用于量化miRNA-21.1.
使用金属有机框架 (MOF) 作为原子转移激素聚合 (ATRP) 的催化剂.
为了在生物样本中实现miRNA-21的超敏感检测.

主要方法:

一个用聚 (diallyldimethylammonium chloride) (PDDA) 和Mn-多孔协调网络 (Mn-PCN-222) 修改的氧化物 (ITO) 电极的制造.
用于miRNA-21识别的氨基化ssDNA (NH2-DNA) 的固定.
使用Fc-DNA@β-CD-Br15和由Mn-PCN-222.2催化的铁甲基酸盐 (FcMMA) 启动ATRP.
聚合FcMMA信号的电化学检测.

主要成果:

开发的生物传感器证明了miRNA-21的超敏感检测,检测极限为0.4 fM.
Mn-PCN-222 MOF 作为ATRP的有效绿色催化剂.
生物传感器在人血清样本中成功检测到miRNA-21.
FcMMA聚合物的电化学信号在~0.33V时是明显的.

结论:

MOF催化ATRP方法为miRNA检测提供了一个敏感和绿色的平台.
这种电化学生物传感器显示出与miRNA-21相关的疾病早期临床诊断的巨大潜力.
该战略强调了MOF在开发先进生物传感技术中的实用性.

关键词:

在ATRP中使用ATRP.主机与客人的互动.MOF MOF MOF MOF MOF MOF MOF MOF MOF MOF MOF MOF MOF MOF MOF MOF MOF MOF MOF MOF MOF MOF 方形波电压测量方形波电压测量这就是miRNA-21-21的原因.这就是β-CD-Br15Br15的特征.

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