基因改造小鼠和其他生物在孟德尔遗传后的繁殖中进行群体规模规划
Vladislava Milchevskaya1,2, Philippe Bugnon3, Emiel B J Ten Buren3
1Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany.
Lab animal
|July 24, 2023
概括
本研究介绍了一个R包和网站,以优化转基因动物育种策略. 该工具通过计算必要的繁殖来实现所需的结果,尽量减少动物的使用和成本,并遵守动物福利原则.
科学领域:
- 动物遗传学和动物繁殖
- 生物信息学和计算生物学
- 实验室动物科学 动物科学
背景情况:
- 转基因动物的殖民地管理是资源密集的,受遗传和繁殖变异的影响.
- 有效的育种策略对于尽量减少动物使用至关重要,与动物福利的3R原则保持一致.
研究的目的:
- 开发一个R包和交互式网站,以优化转基因动物殖民地中的育种设计.
- 提供一个计算特定结果所需的最佳繁殖次数的工具,考虑到特定物种的特征和成功概率.
主要方法:
- 开发一个R包和一个公开可访问的互动网站.
- 纳入物种,菌株,系属性和所需成功概率的参数.
- 包括用于规划均衡的性别比或单性别实验的功能.
主要成果:
- 开发的软件优化了育种设计,减少了时间,成本和动物数量.
- 它提供了关于为定义结果所需的繁殖次数的准确信息.
- 分析表明,单性实验需要至少两倍的繁殖,而不是使用所有后代.
结论:
- 该R包和网站提供了有效和道德的转基因动物殖民地管理的宝贵工具.
- 该软件可以适应各种物种,超出了默认的实验室老鼠参数.
- 这个工具支持研究人员通过优化资源配置和尽量减少动物使用来坚持3Rs.
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