微RNA-668-3p通过准Appl1来抑制肌细胞增殖和分化
Haigang Cao1, Tianning Du1,2, Chenchen Li1
1Laboratory of Animal Fat Deposition and Muscle Development, Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
BMC genomics
|July 24, 2023
概括
微RNA-668-3p (miR-668-3p) 通过降低Appl1的调节来抑制骨肌肉的生长,Appl1是肌细胞增殖和分化的关键促进者. 这揭示了肌肉发育中的新型调节途径.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 身体生理学 身体生理学
背景情况:
- 骨肌肉对于运动,新陈代谢和平衡至关重要.
- 肌肉发育涉及肌细胞增殖,融合和分化.
- 微RNAs (miRNAs) 是骨肌肉生长的关键调节者,在这种组织中发现miR-668-3p的高度表达.
研究的目的:
- 研究miR-668-3p在骨肌肉发育中的作用.
- 确定miR-668-3p的目标基因并阐明其作用机制.
主要方法:
- 使用miR-668-3p模仿剂/抑制剂感染了C2C12细胞核.
- 目标基因的mRNA和蛋白质水平使用RT-qPCR和西式涂抹进行了评估.
- 双 luciferase 测定证实了 Appl1 的 miR-668-3p.p. 的向.
主要成果:
- 发现miR-668-3p可以抑制肌细胞增殖和肌细胞分化.
- 与PH域和氨酸拉链1 (Appl1) 相互作用的氨酸被确定为miR-668-3p的直接目标.
- Appl1通过p38 MAPK通路促进肌细胞增殖和分化,其表达挽救了miR-668-3p的抑制作用.
结论:
- 确定了一种涉及 miR-668-3p/Appl1/p38 MAPK 途径在骨肌肉发育中的新型调节机制.
- 这一途径为肌肉生长和分化分子调节提供了新的见解.
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