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用传输电子显微镜对球形生物矿物质进行空间表征的新型聚焦离子束提升孔

Neerja Zambare¹, Bruce Arey², Odeta Qafoku¹

¹Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 902 Battelle Blvd, Richland, WA 99354, USA.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

|July 25, 2023

概括

一种用于聚焦离子束 (FIB) 片制备的新方法允许研究脆弱生物原体样本的整个截面. 这项技术揭示了细菌沉的水矿石中的自光,为微观生物矿物化提供了洞察力.

科学领域:

材料科学材料科学材料科学
生物地质化学生物地质化学
显微镜的使用方法

背景情况:

聚焦离子束 (FIB) 是准备用于电子和X射线分析的薄样本部分 (拉米拉) 的标准.
传统的FIB方法通常只分析样本中的一个小子区域.
生物样本,如多孔碳酸微沉积物,由于其脆弱性而存在独特的挑战.

研究的目的:

开发一种新的FIB片制备技术,用于检查微观,脆弱样品的整个截面.
为了改善传输电子显微镜 (TEM) 网格安装的叶片方向和粘附性.
探索这种方法在研究生物矿物化过程中的潜力.

主要方法:

在球形,多孔,生物沉碳酸上开发并演示了一种新的FIB片制备方案.
该方法确保了FIB后分析,包括TEM的适当的叶片方向.
促进了叶片对TEM电网柱的安全粘附.

主要成果:

具有挑战性的生物原始样本的整个截面成功地准备和分析.
在使用FIB-SEM和TEM选择区域衍射的细菌沉瓦特里特中观察到自身光.
这种方法对于脆弱,多孔和空洞的微沉物是有效的.

结论:

这种创新的FIB片制备方法可以对整个脆弱样本进行全面的微观结构分析.
它促进了微到纳米尺度生物矿物化的研究,为细菌微环境反应提供了洞察力.
在瓦特里特中检测出自光,为研究生物性矿物形成开辟了新的途径.

关键词:

在现场升降在FIB的拉梅拉.生物矿物质生物矿物质这些微粒是球形的微粒.瓦特里特的自发光是一种自发光.

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