基于核磁共振的溶剂系统选择用于反流色谱分离同一高性能液体色谱峰值中存在的化合物:大麦幼苗中的黄胺作为一个例子
Tao Chen1,2, Jing Jia3, Denglang Zou4
1Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, P.R. China.
Journal of separation science
|July 25, 2023
概括
核磁共振 (NMR) 技术可以在同一高性能液态染色学 (HPLC) 峰值中对化合物进行分区系数选. 这有助于反流色谱分离共同排泄化合物,如密切相关的黄类化合物.
科学领域:
- 分析化学 分析化学
- 染色体学 染色体学 是一种染色学.
- 频谱学是一种光谱学.
背景情况:
- 分割系数对于逆流色谱 (CCC) 分离至关重要.
- 高性能液体染色学 (HPLC) 是选分区系数的标准.
- 在HPLC中,化合物在相同的峰值中排泄.
研究的目的:
- 为了证明核磁共振 (NMR) 技术在选共化化合物的分离系数方面的潜力.
- 开发一种使用NMR选CCC溶剂系统的新策略.
- 扩大CCC的应用范围.
主要方法:
- 利用NMR技术来选HPLC峰值中共排泄的化合物的分区系数.
- 采用NMR来识别CCC的优化溶剂系统:乙烯酸/n-butanol/水 (7:3:10,v/v/v).
- 使用CCC与NMR选溶剂系统分离了两个密切相关的黄类.
主要成果:
- 在相同的HPLC峰值中的化合物中使用NMR成功选分区系数.
- 从一个15毫克的混合物中分离了两个黄化合物,3'-甲-6''-O-feruloylsaponarin (4.8毫克) 和6''-O-feruloylsaponarin (9.8毫克).
- 证明了分离具有轻微结构差异的化合物的可行性 (例如,一个甲基组).
结论:
- 核磁共振技术是选分区系数的强大工具,特别是在联合排泄化合物中.
- 开发的基于NMR的策略增强了CCC溶剂系统查.
- 这种方法扩大了CCC在复杂混合物分离中的实用性.
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