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Updated: Jul 22, 2025

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
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从Cryptococcus neoformans的指导进化中获得的CPE中蛋白的高效拼接PRP8中蛋白

Qin Zhan1, Changhua Shi1, Yu Jiang1

  • 1College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China.

Acta biochimica et biophysica Sinica
|July 25, 2023
PubMed
概括

研究人员设计了一种用于蛋白质拼接的新型CPE蛋白质,实现了比野生类型高29倍的拼接率. 蛋白质工程的进步为未来的应用提供了更高的效率和特异性.

关键词:
这就是C-extein.在PRP8中没有任何内容.指导进化是指导进化的.

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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