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机械方法研究周细胞层的分子结构

Nadezda Makarova¹, Małgorzata Lekka², Kajangi Gnanachandran²

¹Department of Mechanical Engineering, Tufts University, Medford, Massachusetts 02155, United States.

ACS applied materials & interfaces

|July 25, 2023

概括

这项研究揭示了癌细胞已经改变了细胞周层,影响了机械特性. 使用原子力显微镜 (AFM) 了解这些层,为细胞生物学提供了新的见解.

科学领域:

生物物理学的生物物理.
细胞生物学细胞生物学
癌症研究癌症研究

背景情况:

原子力显微镜 (AFM) 用于研究细胞的机械特性.
与非恶性细胞相比,癌细胞经常表现出改变的机械特性.
细胞周层显著影响细胞硬度的测量,但经常被忽视.

研究的目的:

使用AFM调查膀癌细胞 (TCCSUP) 和非恶性细胞 (HCV29) 的机械特性.
使用刷模型将细胞周层纳入AFM分析.
确定恶性和非恶性细胞中细胞周层的物理和生物化学特性.

主要方法:

使用原子力显微镜 (AFM) 和刷模型进行细胞缩研究.
分析了膀上皮非恶性 (HCV29) 和癌症 (TCCSUP) 细胞.
应用了酶治疗方法 (氨酶I,神经氨基酶) 来探测细胞周层的组成.

主要成果:

与非恶性细胞相比,癌细胞表现出更长的内围细胞刷,但与非恶性细胞相比,移植密度相似.
在癌细胞中,外围细胞周边的刷子较短,密度较小.
开发了一种方法,将细胞周层的物理特性转化为生物化学术语.

结论:

周细胞层的物理性质在恶性和非恶性膀细胞之间有所不同.
考虑细胞周层的AFM分析,为细胞力学提供了更全面的理解.
开发的方法有助于破译细胞层的分子组成.

关键词:

一个刷子模型模型.细胞力学细胞力学酶处理,原子力显微镜 (AFM)周细胞层是周细胞层.泌尿器和膀癌,尿道癌和膀癌.

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