两个RNA结合蛋白调节了miR223从线粒体到外体的分类
Liang Ma1, Jasleen Singh1, Randy Schekman1
1Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, United States.
eLife
|July 25, 2023
概括
线粒体储存microRNA-223 (miR223),它被Y盒结合蛋白1 (YBX1) 调动用于外体分泌. YBAP1负面调节了这个过程,影响了线粒体和外体中的miR223水平.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
背景情况:
- 外体是分泌的囊泡,参与细胞间通信.
- 像YBX1这样的RNA结合蛋白调节了特定RNA的分类到外体.
- 线粒体在外体包装之前在RNA的细胞质局部化中发挥作用.
研究的目的:
- 为了研究miR223分类成外体的机制.
- 为了确定参与调节miR223局部化和外体包装的蛋白质.
- 阐明线粒体在外体生物发生和RNA出口中的作用.
主要方法:
- 用RNA结合测试来确定miR223-YBX1的相互作用.
- 活细胞成像用于追踪YBX1的定位.
- 在YBAP1操纵后对线粒体和外体中的miR223水平的分析.
主要成果:
- miR223通过一个UCAGU基因特异地与YBX1的冷震域结合.
- 细胞质miR223在外体组分类之前主要局部化在线粒体中.
- 失去YBAP1会导致线粒体miR223减少和外体miR223.3增加.
- YBX1在线粒体和内分体之间穿,并将其分成P体.
结论:
- 线粒体作为miR223和潜在的其他miRNA的储存库.
- YBX1有助于从线粒体中调动miR223进行外体包装.
- YBAP1作为miR223外体丰富的负调节剂.
- 提出了一个模型,YBX1将线粒体miR223运送到细胞质颗粒中,用于外体体内.
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