针对第一个尼日利亚基因型I ASFV基因组的目标捕获测序
Adeniyi C Adeola1,2,3, Pam D Luka4, Xiang-Xiang Jiang1,5
1State Key Laboratory of Genetic Resources and Evolution and Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, PR China.
Microbial genomics
|July 25, 2023
概括
来自尼日利亚的非洲猪瘟病毒 (ASFV) 基因组首次被测序. 这些发现揭示了与贝宁97/1分离物密切相关的ASFV I基因型菌株,有助于理解病毒演变.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子流行病学分子流行病学
- 基因组学就是基因组学.
背景情况:
- 非洲猪瘟 (ASF) 在猪群中造成了重大的全球经济损失.
- 自1997年以来,尼日利亚经常出现ASF疫情.
- 了解ASFV的基因型和家族遗传关系对于疾病控制至关重要.
研究的目的:
- 确定尼日利亚两种ASFV分离的近乎完整的基因组.
- 进行尼日利亚ASFV分离体的全基因组遗传学分析.
- 阐明尼日利亚ASFV菌株的起源和进化关系.
主要方法:
- 在2016年收集的尼日利亚土著猪样本中检测到ASFV.
- 使用溶液中混合捕获测序,组装近乎完整的ASFV基因组.
- 使用p72基因和全基因组数据进行全基因组的遗传学分析.
主要成果:
- 从150个尼日利亚猪样本中确定了两个ASFV阳性样本.
- 两个尼日利亚ASFV基因组被分配到I基因型,与贝宁97/1菌株密切相关.
- 在尼日利亚分离物和贝宁 97/1.1.之间观察到高基因组序列一致性 (99.94%和99.92%).
结论:
- 这项研究为尼日利亚的ASFV提供了第一个几乎完整的基因组序列.
- 这些发现表明,尼日利亚的ASFV分离物和来自贝宁和意大利的菌株之间存在密切的关系.
- 溶液中混合捕获测序是一种有效的ASFV基因组测定方法,有助于更好地了解ASFV的全球演变.
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